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Non-invasive in vivo quantification of the developing optical properties and graded index of the embryonic eye lens using SPIM.

Young, L.K. and Jarrin, M. and Saunter, C.D. and Quinlan, R.A. and Girkin, J.M. (2018) 'Non-invasive in vivo quantification of the developing optical properties and graded index of the embryonic eye lens using SPIM.', Biomedical optics express., 9 (5). pp. 2176-2188.


Graded refractive index lenses are inherent to advanced visual systems in animals. By understanding their formation and local optical properties, significant potential for improved ocular healthcare may be realized. We report a novel technique measuring the developing optical power of the eye lens, in a living animal, by exploiting the orthogonal imaging modality of a selective plane illumination microscope (SPIM). We have quantified the maturation of the lenticular refractive index at three different visible wavelengths using a combined imaging and ray tracing approach. We demonstrate that the method can be used with transgenic and vital dye labeling as well as with both fixed and living animals. Using a key eye lens morphogen and its inhibitor, we have measured their effects both on lens size and on refractive index. Our technique provides insights into the mechanisms involved in the development of this natural graded index micro-lens and its associated optical properties.

Item Type:Article
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Publisher statement:Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article's title, journal citation, and DOI.
Date accepted:23 March 2018
Date deposited:28 March 2018
Date of first online publication:10 April 2018
Date first made open access:No date available

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