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Enhancing multi-spot structured illumination microscopy with fluorescence difference.

Ward, Edward N. and Torkelsen, Frida H. and Pal, Robert (2018) 'Enhancing multi-spot structured illumination microscopy with fluorescence difference.', Royal Society open science., 5 (3). p. 171336.


Structured illumination microscopy is a super-resolution technique used extensively in biological research. However, this technique is limited in the maximum possible resolution increase. Here we report the results of simulations of a novel enhanced multi-spot structured illumination technique. This method combines the super-resolution technique of difference microscopy with structured illumination deconvolution. Initial results give at minimum a 1.4-fold increase in resolution over conventional structured illumination in a low-noise environment. This new technique also has the potential to be expanded to further enhance axial resolution with three-dimensional difference microscopy. The requirement for precise pattern determination in this technique also led to the development of a new pattern estimation algorithm which proved more efficient and reliable than other methods tested.

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Publisher statement:© 2018 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License, which permits unrestricted use, provided the original author and source are credited.
Date accepted:09 February 2018
Date deposited:29 March 2018
Date of first online publication:14 March 2018
Date first made open access:29 March 2018

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