Ward, Edward N. and Torkelsen, Frida H. and Pal, Robert (2018) 'Enhancing multi-spot structured illumination microscopy with fluorescence difference.', Royal Society open science., 5 (3). p. 171336.
Abstract
Structured illumination microscopy is a super-resolution technique used extensively in biological research. However, this technique is limited in the maximum possible resolution increase. Here we report the results of simulations of a novel enhanced multi-spot structured illumination technique. This method combines the super-resolution technique of difference microscopy with structured illumination deconvolution. Initial results give at minimum a 1.4-fold increase in resolution over conventional structured illumination in a low-noise environment. This new technique also has the potential to be expanded to further enhance axial resolution with three-dimensional difference microscopy. The requirement for precise pattern determination in this technique also led to the development of a new pattern estimation algorithm which proved more efficient and reliable than other methods tested.
| Item Type: | Article |
|---|---|
| Full text: | (VoR) Version of Record Available under License - Creative Commons Attribution. Download PDF (783Kb) |
| Status: | Peer-reviewed |
| Publisher Web site: | https://doi.org/10.1098/rsos.171336 |
| Publisher statement: | © 2018 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited. |
| Date accepted: | 09 February 2018 |
| Date deposited: | 29 March 2018 |
| Date of first online publication: | 14 March 2018 |
| Date first made open access: | 29 March 2018 |
Save or Share this output
| Export: | |
| Look up in GoogleScholar |
