Ward, Edward N. and Torkelsen, Frida H. and Pal, Robert (2018) 'Enhancing multi-spot structured illumination microscopy with fluorescence difference.', Royal Society open science., 5 (3). p. 171336.
Structured illumination microscopy is a super-resolution technique used extensively in biological research. However, this technique is limited in the maximum possible resolution increase. Here we report the results of simulations of a novel enhanced multi-spot structured illumination technique. This method combines the super-resolution technique of difference microscopy with structured illumination deconvolution. Initial results give at minimum a 1.4-fold increase in resolution over conventional structured illumination in a low-noise environment. This new technique also has the potential to be expanded to further enhance axial resolution with three-dimensional difference microscopy. The requirement for precise pattern determination in this technique also led to the development of a new pattern estimation algorithm which proved more efficient and reliable than other methods tested.
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|Publisher Web site:||https://doi.org/10.1098/rsos.171336|
|Publisher statement:||© 2018 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.|
|Date accepted:||09 February 2018|
|Date deposited:||29 March 2018|
|Date of first online publication:||14 March 2018|
|Date first made open access:||29 March 2018|
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