Cookies

We use cookies to ensure that we give you the best experience on our website. By continuing to browse this repository, you give consent for essential cookies to be used. You can read more about our Privacy and Cookie Policy.


Durham Research Online
You are in:

Heat shock proteins are differentially expressed in brain and spinal cord : implications for multiple sclerosis.

Gorter, Rianne P. and Nutma, Erik and Jahreiβ, Marie-Christina and de Jonge, Jenny C. and Quinlan, Roy and van der Valk, Paul and van Noort, Johannes M. and Baron, Wia and Amor, Sandra (2018) 'Heat shock proteins are differentially expressed in brain and spinal cord : implications for multiple sclerosis.', Clinical and experimental immunology., 194 (2). pp. 137-152.

Abstract

Aims: Multiple sclerosis (MS) is a chronic neurodegenerative disease characterised by demyelination, inflammation and neurodegeneration throughout the central nervous system. Although spinal cord pathology is an important factor contributing to disease progression, few studies have examined MS lesions in the spinal cord and how they differ from brain lesions. Here we have compared brain and spinal cord white and grey matter from MS and control tissues focussing on small heat shock proteins (HSPB) and HSP16.2. Methods: Western blotting was used to examine protein levels of HSPB1, HSPB5, HSPB6, HSPB8 and HSP16.2 in brain and spinal cord from MS and age‐matched non‐neurological controls. Immunohistochemistry was used to examine expression of the HSPs in MS spinal cord lesions and controls. Expression levels were quantified using ImageJ. Results: Western blotting revealed significantly higher levels of HSPB1, HSPB6 and HSPB8 in MS and control spinal cord compared to brain tissues. No differences in HSPB5 and HSP16.2 protein levels were observed although HSPB5 protein levels were higher in brain WM versus GM. In MS spinal cord lesions, increased HSPB1 and HSPB5 expression was observed in astrocytes, and increased neuronal expression of HSP16.2 was observed in normal appearing grey matter and type 1 grey matter lesions. Conclusions: The high constitutive expression of several HSPBs in spinal cord and increased expression of HSPBs and HSP16.2 in MS illustrate differences between brain and spinal cord in health and upon demyelination. Regional differences in HSP expression may reflect differences in astrocyte cytoskeleton composition and influence inflammation, possibly affecting the effectiveness of pharmacological agents.

Item Type:Article
Full text:Publisher-imposed embargo
(AM) Accepted Manuscript
Available under License - Creative Commons Attribution Non-commercial No Derivatives.
File format - PDF
(1816Kb)
Full text:(VoR) Version of Record
Available under License - Creative Commons Attribution Non-commercial No Derivatives.
Download PDF
(1228Kb)
Status:Peer-reviewed
Publisher Web site:https://doi.org/10.1111/cei.13186
Publisher statement:© 2018 The Authors. Clinical & Experimental Immunology published by John Wiley & Sons Ltd on behalf of British Society for Immunology. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
Date accepted:11 July 2018
Date deposited:23 July 2018
Date of first online publication:19 September 2018
Date first made open access:No date available

Save or Share this output

Export:
Export
Look up in GoogleScholar