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Single-nanoparticle cell barcoding by tunable FRET from lanthanides to quantum dots.

Chen, Chi and Ao, Lijiao and Wu, Yu-Tang and Cifliku, Vjona and Cardoso Dos Santos, Marcelina and Bourrier, Emmanuel and Delbianco, Martina and Parker, David and Zwier, Jurriaan and Huang, Liang and Hildebrandt, Niko (2018) 'Single-nanoparticle cell barcoding by tunable FRET from lanthanides to quantum dots.', Angewandte Chemie., 130 (41). pp. 13876-13881.


Fluorescence barcoding based on nanoparticles provides many advantages for multiparameter imaging. However, creating different concentration‐independent codes without mixing various nanoparticles and by using single wavelength excitation and emission for multiplexed cellular imaging is extremely challenging. Here, we report the development of quantum dots (QDs) with two different SiO2 shell thicknesses (6 and 12 nm) and coated with two different lanthanide complexes (Tb and Eu). FRET from Tb or Eu donors to the QD acceptors resulted in four distinct photoluminescence (PL) decays, which were encoded by simple time‐gated (TG) PL intensity detection in three individual temporal detection windows. The well‐defined single‐nanoparticle codes were used for live cell imaging and a one‐measurement distinction of four different cells in a single field of view. This single‐color barcoding strategy opens new opportunities for multiplexed labeling and tracking of cells.

Item Type:Article
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Publisher statement:This is the accepted version of the following article: Chen, Chi, Ao, Lijiao, Wu, Yu-Tang, Cifliku, Vjona, Cardoso Dos Santos, Marcelina, Bourrier, Emmanuel, Delbianco, Martina, Parker, David, Zwier, Jurriaan, Huang, Liang & Hildebrandt, Niko (2018). Single-Nanoparticle Cell Barcoding by Tunable FRET from Lanthanides to Quantum Dots. Angewandte Chemie 130(41): 13876-13881 which has been published in final form at This article may be used for non-commercial purposes in accordance With Wiley-VCH Terms and Conditions for self-archiving.
Date accepted:07 August 2018
Date deposited:08 August 2018
Date of first online publication:28 August 2018
Date first made open access:28 August 2019

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