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Mitochondrial ATP production provides long-range control of endothelial inositol trisphosphate–evoked calcium signaling.

Wilson, Calum and Lee, Matthew D. and Heathcote, Helen and Zhang, Xun and Buckley, Charlotte and Girkin, John M. and Saunter, Christopher D. and McCarron, John G. (2019) 'Mitochondrial ATP production provides long-range control of endothelial inositol trisphosphate–evoked calcium signaling.', Journal of biological chemistry., 294 (3). p. 758.

Abstract

Endothelial cells are reported to be glycolytic and to minimally rely on mitochondria for ATP generation. Rather than providing energy, mitochondria in endothelial cells may act as signaling organelles that control cytosolic Ca2+ signaling or modify reactive oxygen species (ROS). To control Ca2+ signaling, these organelles are often observed close to influx and release sites and may be tethered near Ca2+ transporters. In this study, we used high-resolution, wide-field fluorescence imaging to investigate the regulation of Ca2+ signaling by mitochondria in large numbers of endothelial cells (~50 per field) in intact arteries from rats. We observed that mitochondria were mostly spherical or form short rod structures and were distributed widely throughout the cytoplasm. The density of these organelles did not increase near contact sites with smooth muscle cells. However, local inositol trisphosphate (IP3)-mediated Ca2+ signaling predominated near these contact sites and required polarized mitochondria. Of note, mitochondrial control of Ca2+ signals occurred even when mitochondria were far from Ca2+ release sites. Indeed, the endothelial mitochondria were mobile and moved throughout the cytoplasm. Mitochondrial control of Ca2+ signaling was mediated by ATP production, which, when reduced by mitochondrial depolarization or ATP synthase inhibition, eliminated local IP3-mediated Ca2+ release events. ROS buffering did not significantly alter local Ca2+ release events. These results highlight the importance of mitochondrial ATP production in providing long-range control of endothelial signaling via IP3-evoked local Ca2+ release in intact endothelium.

Item Type:Article
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Status:Peer-reviewed
Publisher Web site:https://doi.org/10.1074/jbc.RA118.005913
Publisher statement:This research was originally published in Wilson, Calum, Lee, Matthew D., Heathcote, Helen, Zhang, Xun, Buckley, Charlotte, Girkin, John M., Saunter, Christopher D. & McCarron, John G. (2019). Mitochondrial ATP production provides long-range control of endothelial inositol trisphosphate–evoked calcium signaling. Journal of Biological Chemistry 294(3): 737-758. Final version published open access under the terms of the Creative Commons CC-BY license.
Date accepted:27 November 2018
Date deposited:05 December 2018
Date of first online publication:29 November 2018
Date first made open access:No date available

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