We use cookies to ensure that we give you the best experience on our website. By continuing to browse this repository, you give consent for essential cookies to be used. You can read more about our Privacy and Cookie Policy.

Durham Research Online
You are in:

Holographic projection for multi-spot structured illumination microscopy.

Ward, E. N. and Pal, R. (2019) 'Holographic projection for multi-spot structured illumination microscopy.', Journal of microscopy., 274 (2). pp. 114-120.


This paper describes the design and testing of a multi‐spot structured illumination microscopy system using computer‐generated holograms to create the required excitation patterns. Furthermore, it demonstrates the use of an adapted direct search algorithm for calculating the holograms that allows for imaging across an extended field of view. The system was tested on fixed targets and live cells yielding a two times resolution increase over conventional diffraction‐limited imaging.

Item Type:Article
Full text:(AM) Accepted Manuscript
Download PDF
Publisher Web site:
Publisher statement:This is the accepted version of the following article: Ward, E. N. & Pal, R. (2019). Holographic projection for multi-spot structured illumination microscopy. Journal of Microscopy 274(2): 114-120 which has been published in final form at This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
Date accepted:19 February 2019
Date deposited:22 February 2019
Date of first online publication:21 February 2019
Date first made open access:21 February 2020

Save or Share this output

Look up in GoogleScholar