Orosa-Puente, Beatriz and Leftley, Nicola and von Wangenheim, Daniel and Banda, Jason and Srivastava, Anjil K. and Hill, Kristine and Truskina, Jekaterina and Bhosale, Rahul and Morris, Emily and Srivastava, Moumita and Kümpers, Britta and Goh, Tatsuaki and Fukaki, Hidehiro and Vermeer, Joop E. M. and Vernoux, Teva and Dinneny, José R. and French, Andrew P. and Bishopp, Anthony and Sadanandom, Ari and Bennett, Malcolm J. (2018) 'Root branching toward water involves posttranslational modification of transcription factor ARF7.', Science., 362 (6421). pp. 1407-1410.
Abstract
Plants adapt to heterogeneous soil conditions by altering their root architecture. For example, roots branch when in contact with water using the hydropatterning response. We report that hydropatterning is dependent on auxin response factor ARF7. This transcription 5 factor induces asymmetric expression of its target gene LBD16 in lateral root founder cells on the side of the root in contact with water. This differential expression pattern is regulated by post-translational modification of ARF7 with the SUMO protein. SUMOylation negatively regulates ARF7 DNA binding activity. ARF7 SUMOylation is required to recruit the Aux/IAA repressor protein IAA3. Blocking ARF7 SUMOylation disrupts IAA3 10 recruitment and hydropatterning. We conclude that SUMO-dependent regulation of auxin response controls root branching pattern in response to water availability.
| Item Type: | Article |
|---|---|
| Full text: | (AM) Accepted Manuscript Download PDF (743Kb) |
| Status: | Peer-reviewed |
| Publisher Web site: | https://doi.org/10.1126/science.aau3956 |
| Publisher statement: | This is the author’s version of the work. It is posted here by permission of the AAAS for personal use, not for redistribution. The definitive version was published in [Orosa-Puente, Beatriz, Leftley, Nicola, von Wangenheim, Daniel, Banda, Jason, Srivastava, Anjil K., Hill, Kristine, Truskina, Jekaterina, Bhosale, Rahul, Morris, Emily, Srivastava, Moumita, Kümpers, Britta, Goh, Tatsuaki, Fukaki, Hidehiro, Vermeer, Joop E. M., Vernoux, Teva, Dinneny, José R., French, Andrew P., Bishopp, Anthony, Sadanandom, Ari & Bennett, Malcolm J. (2018). Root branching toward water involves posttranslational modification of transcription factor ARF7. Science 362(6421): 1407-1410. DOI: 10.1126/science.aau3956 |
| Date accepted: | 06 November 2018 |
| Date deposited: | 08 March 2019 |
| Date of first online publication: | 21 December 2018 |
| Date first made open access: | 08 March 2019 |
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