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Pulling force deforms hair follicle root sheath nuclei and surrounding dermal collagen matrix differently at infundibulum, isthmus and suprabulbar regions.

Johnson, Simeon and Cowley, Kevin and Hawkins, Timothy J. and Määttä, Arto (2019) 'Pulling force deforms hair follicle root sheath nuclei and surrounding dermal collagen matrix differently at infundibulum, isthmus and suprabulbar regions.', Experimental dermatology., 28 (7). pp. 862-866.

Abstract

The biomechanical properties of the collagenous dermal matrix are well described but responses to mechanical force by the hair follicles have not been characterised so far. We applied a pulling force on hair follicles to visualise and quantify changes in the keratin‐14 and involucrin positive cell layers of the follicles using nuclear dimensions as an indicator of tissue deformation. Moreover, we used second harmonic generation imaging to visualise changes in the dermal collagen. We report how the anatomical regions of the follicle respond to the force. Nuclei of the isthmus region were most affected. The nuclei in both K14 positive outer root sheath cells and in involucrin positive cells were significantly compressed, whereas the response in the infundibulum and suprabulbar regions was more variable. The deformation of the nuclei did not correlate with lamin A/C expression. The changes in the collagenous matrix were distinct at different depths of the dermis as collagen fibrils were compressed closer to each other in the region adjacent to upper suprabulbar follicle and pulled apart near the infundibulum. Thus, the responses to the force are locally defined and the cells in the permanent and cycling parts of the follicle behave differently.

Item Type:Article
Full text:(AM) Accepted Manuscript
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Status:Peer-reviewed
Publisher Web site:https://doi.org/10.1111/exd.13948
Publisher statement:This is the accepted version of the following article: Johnson, Simeon, Cowley, Kevin, Hawkins, Timothy J. & Määttä, Arto (2019). Pulling force deforms hair follicle root sheath nuclei and surrounding dermal collagen matrix differently at infundibulum, isthmus and suprabulbar regions. Experimental Dermatology 28(7): 862-866., which has been published in final form at https://doi.org/10.1111/exd.13948. This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
Date accepted:16 April 2019
Date deposited:26 April 2019
Date of first online publication:25 April 2019
Date first made open access:25 April 2020

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