Split-QF System for Fine-Tuned Transgene Expression in Drosophila

Riabinina, O.; Vernon, S.W.; Dickson, B.J.; Baines, R.A.

doi:10.1534/genetics.119.302034

Split-QF System for Fine-Tuned Transgene Expression in Drosophila

Riabinina, O.; Vernon, S.W.; Dickson, B.J.; Baines, R.A.

Authors

Dr Lena Riabinina olena.riabinina@durham.ac.uk
Associate Professor

S.W. Vernon

B.J. Dickson

R.A. Baines

Abstract

The Q-system is a binary expression system that works well across species. Here, we report the development and demonstrate the applications of a split-QF system that drives strong expression in Drosophila, is repressible by QS, and is inducible by a small nontoxic molecule (quinic acid). The split-QF system is fully compatible with existing split-GAL4 and split-LexA lines, thus greatly expanding the range of possible advanced intersectional experiments and anatomical, physiological, and behavioral assays in Drosophila, and in other organisms.

Citation

Riabinina, O., Vernon, S., Dickson, B., & Baines, R. (2019). Split-QF System for Fine-Tuned Transgene Expression in Drosophila. Genetics, 212(1), 53-63. https://doi.org/10.1534/genetics.119.302034

Journal Article Type	Article
Acceptance Date	Mar 3, 2019
Online Publication Date	May 3, 2019
Publication Date	May 31, 2019
Deposit Date	May 16, 2019
Publicly Available Date	May 22, 2019
Journal	Genetics
Print ISSN	0016-6731
Electronic ISSN	1943-2361
Publisher	Genetics Society of America
Peer Reviewed	Peer Reviewed
Volume	212
Issue	1
Pages	53-63
DOI	https://doi.org/10.1534/genetics.119.302034

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Copyright Statement
Copyright © 2019 Riabinina et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.