Expression levels of inositol phosphorylceramide synthase modulate plant responses to biotic and abiotic stress in Arabidopsis thaliana

Pinneh, E.C.; Stoppel, R.; Knight, H.; Knight, M.R.; Steel, P.G.; Denny, P.W.

doi:10.1371/journal.pone.0217087

Expression levels of inositol phosphorylceramide synthase modulate plant responses to biotic and abiotic stress in Arabidopsis thaliana

Pinneh, E.C.; Stoppel, R.; Knight, H.; Knight, M.R.; Steel, P.G.; Denny, P.W.

Authors

E.C. Pinneh

R. Stoppel

Professor Heather Knight p.h.knight@durham.ac.uk
Professor

Professor Marc Knight m.r.knight@durham.ac.uk
Professor

P.G. Steel

Professor Paul Denny p.w.denny@durham.ac.uk
Professor

Abstract

This research was undertaken to investigate the global role of the plant inositol phosphorylceramide synthase (IPCS), a non-mammalian enzyme previously shown to be associated with the pathogen response. RNA-Seq analyses demonstrated that over-expression of inositol phosphorylceramide synthase isoforms AtIPCS1, 2 or 3 in Arabidopsis thaliana resulted in the down-regulation of genes involved in plant response to pathogens. In addition, genes associated with the abiotic stress response to salinity, cold and drought were found to be similarly down-regulated. Detailed analyses of transgenic lines over-expressing AtIPCS1-3 at various levels revealed that the degree of down-regulation is specifically correlated with the level of IPCS expression. Singular enrichment analysis of these down-regulated genes showed that AtIPCS1-3 expression affects biological signaling pathways involved in plant response to biotic and abiotic stress. The up-regulation of genes involved in photosynthesis and lipid localization was also observed in the over-expressing lines.

Citation

Pinneh, E., Stoppel, R., Knight, H., Knight, M., Steel, P., & Denny, P. (2019). Expression levels of inositol phosphorylceramide synthase modulate plant responses to biotic and abiotic stress in Arabidopsis thaliana. PLoS ONE, 14(5), Article e0217087. https://doi.org/10.1371/journal.pone.0217087

Journal Article Type	Article
Acceptance Date	Apr 30, 2019
Online Publication Date	May 23, 2019
Publication Date	May 23, 2019
Deposit Date	Sep 13, 2018
Publicly Available Date	May 24, 2019
Journal	PLoS ONE
Publisher	Public Library of Science
Peer Reviewed	Peer Reviewed
Volume	14
Issue	5
Article Number	e0217087
DOI	https://doi.org/10.1371/journal.pone.0217087

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Copyright Statement
Copyright: © 2019 Pinneh et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.