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Multiple C2 domains and transmembrane region proteins (MCTPs) tether membranes at plasmodesmata

Brault, Marie L; Petit, Jules D; Immel, Françoise; Nicolas, William J; Glavier, Marie; Brocard, Lysiane; Gaston, Amèlia; Fouché, Mathieu; Hawkins, Timothy J; Crowet, Jean‐Marc; Grison, Magali S; Germain, Véronique; Rocher, Marion; Kraner, Max; Alva, Vikram; Claverol, Stéphane; Paterlini, Andrea; Helariutta, Ykä; Deleu, Magali; Lins, Laurence; Tilsner, Jens; Bayer, Emmanuelle M

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Authors

Marie L Brault

Jules D Petit

Françoise Immel

William J Nicolas

Marie Glavier

Lysiane Brocard

Amèlia Gaston

Mathieu Fouché

Jean‐Marc Crowet

Magali S Grison

Véronique Germain

Marion Rocher

Max Kraner

Vikram Alva

Stéphane Claverol

Andrea Paterlini

Ykä Helariutta

Magali Deleu

Laurence Lins

Jens Tilsner

Emmanuelle M Bayer



Abstract

In eukaryotes, membrane contact sites (MCS) allow direct communication between organelles. Plants have evolved a unique type of MCS, inside intercellular pores, the plasmodesmata, where endoplasmic reticulum (ER)–plasma membrane (PM) contacts coincide with regulation of cell‐to‐cell signalling. The molecular mechanism and function of membrane tethering within plasmodesmata remain unknown. Here, we show that the multiple C2 domains and transmembrane region protein (MCTP) family, key regulators of cell‐to‐cell signalling in plants, act as ER‐PM tethers specifically at plasmodesmata. We report that MCTPs are plasmodesmata proteins that insert into the ER via their transmembrane region while their C2 domains dock to the PM through interaction with anionic phospholipids. A Atmctp3/Atmctp4 loss of function mutant induces plant developmental defects, impaired plasmodesmata function and composition, while MCTP4 expression in a yeast Δtether mutant partially restores ER‐PM tethering. Our data suggest that MCTPs are unique membrane tethers controlling both ER‐PM contacts and cell‐to‐cell signalling.

Citation

Brault, M. L., Petit, J. D., Immel, F., Nicolas, W. J., Glavier, M., Brocard, L., …Bayer, E. M. (2019). Multiple C2 domains and transmembrane region proteins (MCTPs) tether membranes at plasmodesmata. EMBO Reports, 20(8), Article e47182. https://doi.org/10.15252/embr.201847182

Journal Article Type Article
Acceptance Date Jun 6, 2019
Online Publication Date Jul 9, 2019
Publication Date Aug 1, 2019
Deposit Date Jul 11, 2019
Publicly Available Date Jul 24, 2019
Journal EMBO Reports
Print ISSN 1469-221X
Electronic ISSN 1469-3178
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 20
Issue 8
Article Number e47182
DOI https://doi.org/10.15252/embr.201847182

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