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Increase in lysophosphatidate acyltransferase activity in oilseed rape (Brassica napus L.) increases seed triacylglycerol content despite its low intrinsic flux control coefficient.

Woodfield, Helen K. and Fenyk, Stepan and Wallington, Emma and Bates, Ruth E. and Brown, Alexander and Guschina, Irina A. and Marillia, Elizabeth-France and Taylor, David C. and Fell, David and Harwood, John L. and Fawcett, Tony (2019) 'Increase in lysophosphatidate acyltransferase activity in oilseed rape (Brassica napus L.) increases seed triacylglycerol content despite its low intrinsic flux control coefficient.', New phytologist., 224 (2). pp. 700-711.

Abstract

Lysophosphatidate acyltransferase (LPAAT) catalyses the second step of the Kennedy pathway for triacylglycerol (TAG) synthesis. Here we express Trapaeolum majus LPAAT in Brassica napus (B. napus) cv 12075 to evaluate the effects on lipid synthesis and estimate the flux control coefficient for LPAAT. We estimate the flux control coefficient of LPAAT in a whole plant context by deriving a relationship between it and overall lipid accumulation, given that this is an exponential process. Increasing LPAAT activity resulted in greater TAG accumulation in seeds of between 25 and 29%; altered fatty acid distributions in seed lipids (particularly those of the Kennedy pathway); and a redistribution of label from 14C‐glycerol between phosphoglycerides. Greater LPAAT activity in seeds led to an increase in TAG content despite its low intrinsic flux control coefficient on account of the exponential nature of lipid accumulation that amplifies the effect of the small flux increment achieved by increasing its activity. We have also developed a novel application of metabolic control analysis likely to have broad application as it determines the in planta flux control that a single component has upon accumulation of storage products.

Item Type:Article
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Status:Peer-reviewed
Publisher Web site:https://doi.org/10.1111/nph.16100
Publisher statement:© 2019 The Authors. New Phytologist © 2019 New Phytologist Trust. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Date accepted:23 July 2019
Date deposited:06 August 2019
Date of first online publication:14 September 2019
Date first made open access:17 September 2019

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