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MUR1-mediated cell-wall fucosylation is required for freezing tolerance in Arabidopsis thaliana.

Panter, Paige E. and Kent, Olivia and Dale, Maeve and Smith, Sarah J. and Skipsey, Mark and Thorlby, Glenn and Cummins, Ian and Ramsay, Nathan and Begum, Rifat A. and Sanhueza, Dayan and Fry, Stephen C. and Knight, Marc R. and Knight, Heather (2019) 'MUR1-mediated cell-wall fucosylation is required for freezing tolerance in Arabidopsis thaliana.', New phytologist., 224 (4). pp. 1518-1531.


1.Forward genetic screens play a key role in the identification of genes contributing to plant stress tolerance. Using a screen for freezing sensitivity, we have identified a novel freezing tolerance gene, SENSITIVE TO FREEZING8, in Arabidopsis thaliana. 2.We identified SFR8 using recombination‐based mapping and whole‐genome sequencing. As SFR8 was predicted to have an effect on cell wall composition, we used GC‐MS and polyacrylamide gel electrophoresis to measure cell‐wall fucose and boron (B)‐dependent dimerisation of the cell‐wall pectic domain rhamnogalacturonan II (RGII) in planta. After treatments to promote borate‐bridging of RGII, we assessed freeze‐induced damage in wild‐type and sfr8 plants by measuring electrolyte leakage from freeze‐thawed leaf discs. 3.We mapped the sfr8 mutation to MUR1, a gene encoding the fucose biosynthetic enzyme GDP‐d‐mannose‐4,6‐dehydratase. sfr8 cell walls exhibited low cell‐wall fucose levels and reduced RGII bridging. Freezing sensitivity of sfr8 mutants was ameliorated by B supplementation, which can restore RGII dimerisation. B transport mutants with reduced RGII dimerisation were also freezing‐sensitive. 4.Our research identifies a role for the structure and composition of the plant primary cell wall in determining basal plant freezing tolerance and highlights the specific importance of fucosylation, most likely through its effect on the ability of RGII pectin to dimerise.

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Publisher statement:© 2019 The Authors New Phytologist © 2019 New Phytologist Trust. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Date accepted:28 July 2019
Date deposited:06 August 2019
Date of first online publication:07 November 2019
Date first made open access:07 November 2019

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