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Crystal structures of the Bacillus subtilis prophage lytic cassette proteins XepA and YomS.

Freitag-Pohl, Stefanie and Jasilionis, Andrius and Håkansson, Maria and Svensson, L. Anders and Kovačič, Rebeka and Welin, Martin and Watzlawick, Hildegard and Wang, Lei and Altenbuchner, Josef and Płotka, Magdalena and Kaczorowska, Anna Karina and Kaczorowski, Tadeusz and Nordberg Karlsson, Eva and Al-Karadaghi, Salam and Walse, Björn and Aevarsson, Arnthór and Pohl, Ehmke (2019) 'Crystal structures of the Bacillus subtilis prophage lytic cassette proteins XepA and YomS.', Acta crystallographica section D structural biology., 75 (11). pp. 1028-1039.

Abstract

For the extraction of the best possible X-ray diffraction data from macromolecular crystals, accurate positioning of the crystals with respect to the X-ray beam is crucial. In addition, information about the shape and internal defects of crystals allows the optimization of data-collection strategies. Here, it is demonstrated that the X-ray beam available on the macromolecular crystallo­graphy beamline P14 at the high-brilliance synchrotron-radiation source PETRA III at DESY, Hamburg, Germany can be used for high-energy phase-contrast microtomography of protein crystals mounted in an optically opaque lipidic cubic phase matrix. Three-dimensional tomograms have been obtained at X-ray doses that are substantially smaller and on time scales that are substantially shorter than those used for diffraction-scanning approaches that display protein crystals at micrometre resolution. Adding a compound refractive lens as an objective to the imaging setup, two-dimensional imaging at sub-micrometre resolution has been achieved. All experiments were performed on a standard macromolecular crystallography beamline and are compatible with standard diffraction data-collection workflows and apparatus. Phase-contrast X-ray imaging of macromolecular crystals could find wide application at existing and upcoming low-emittance synchrotron-radiation sources.

Item Type:Article
Full text:(VoR) Version of Record
Available under License - Creative Commons Attribution.
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Full text:(VoR) Version of Record
Available under License - Creative Commons Attribution.
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Status:Peer-reviewed
Publisher Web site:https://doi.org/10.1107/S2059798319013330
Publisher statement:This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution and reproduction in any medium, provided the original authors and source are cited.
Date accepted:28 September 2019
Date deposited:13 November 2019
Date of first online publication:November 2019
Date first made open access:13 November 2019

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