Manghwar, H. and Lindsey, K. and Zhang, X. and Jin, S. (2019) 'CRISPR/Cas system : recent advances and prospects for genome editing.', Trends in Plant Science, 24 (12). pp. 1102-1125.
Abstract
Genome editing (GE) has revolutionized biological research through the new ability to precisely edit the genomes of living organisms. In recent years, various GE tools have been explored for editing simple and complex genomes. The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system has widely been used in GE due to its high efficiency, ease of use, and accuracy. It can be used to add desirable and remove undesirable alleles simultaneously in a single event. Here, we discuss various applications of CRISPR/Cas9 in a range of important crops, compare it with other GE tools, and review its mechanism, limitations, and future possibilities. Various newly emerging CRISPR/Cas systems, including base editing (BE), xCas9, and Cas12a (Cpf1), are also considered.
| Item Type: | Article |
|---|---|
| Full text: | (VoR) Version of Record Available under License - Creative Commons Attribution. Download PDF (4404Kb) |
| Status: | Peer-reviewed |
| Publisher Web site: | https://doi.org/10.1016/j.tplants.2019.09.006 |
| Publisher statement: | © 2019 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Date accepted: | No date available |
| Date deposited: | 26 November 2019 |
| Date of first online publication: | 2019 |
| Date first made open access: | 26 November 2019 |
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