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Durham Research Online
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Reactivation of Epstein–Barr virus by a dual-responsive fluorescent EBNA1-targeting agent with Zn2+-chelating function.

Jiang, Lijun and Lung, Hong Lok and Huang, Tao and Lan, Rongfeng and Zha, Shuai and Chan, Lai Sheung and Thor, Waygen and Tsoi, Tik-Hung and Chau, Ho-Fai and Boreström, Cecilia and Cobb, Steven L. and Tsao, Sai Wah and Bian, Zhao-Xiang and Law, Ga-Lai and Wong, Wing-Tak and Tai, William Chi-Shing and Chau, Wai Yin and Du, Yujun and Tang, Lucas Hao Xi and Chiang, Alan Kwok Shing and Middeldorp, Jaap M. and Lo, Kwok-Wai and Mak, Nai Ki and Long, Nicholas J. and Wong, Ka-Leung (2019) 'Reactivation of Epstein–Barr virus by a dual-responsive fluorescent EBNA1-targeting agent with Zn2+-chelating function.', Proceedings of the National Academy of Sciences., 116 (52). pp. 26614-26624.

Abstract

EBNA1 is the only Epstein–Barr virus (EBV) latent protein responsible for viral genome maintenance and is expressed in all EBV-infected cells. Zn2+ is essential for oligomerization of the functional EBNA1. We constructed an EBNA1 binding peptide with a Zn2+ chelator to create an EBNA1-specific inhibitor (ZRL5P4). ZRL5P4 by itself is sufficient to reactivate EBV from its latent infection. ZRL5P4 is able to emit unique responsive fluorescent signals once it binds with EBNA1 and a Zn2+ ion. ZRL5P4 can selectively disrupt the EBNA1 oligomerization and cause nasopharyngeal carcinoma (NPC) tumor shrinkage, possibly due to EBV lytic induction. Dicer1 seems essential for this lytic reactivation. As can been seen, EBNA1 is likely to maintain NPC cell survival by suppressing viral reactivation.

Item Type:Article
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Status:Peer-reviewed
Publisher Web site:https://doi.org/10.1073/pnas.1915372116
Publisher statement:Copyright © 2019 the Author(s). Published by PNAS. This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY).
Date accepted:No date available
Date deposited:15 January 2020
Date of first online publication:10 December 2019
Date first made open access:15 January 2020

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