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Time-resolved luminescence detection of peroxynitrite using a reactivity-based lanthanide probe

Breen, Colum; Pal, Robert; Elsegood, Mark R.J.; Teat, Simon J.; Iza, Felipe; Wende, Kristian; Buckley, Benjamin R.; Butler, Stephen J.

Authors

Colum Breen

Mark R.J. Elsegood

Simon J. Teat

Felipe Iza

Kristian Wende

Benjamin R. Buckley

Stephen J. Butler



Abstract

Peroxynitrite (ONOO−) is a powerful and short-lived oxidant formed in vivo, which can react with most biomolecules directly. To fully understand the roles of ONOO− in cell biology, improved methods for the selective detection and real-time analysis of ONOO− are needed. We present a water-soluble, luminescent europium(III) probe for the rapid and sensitive detection of peroxynitrite in human serum, living cells and biological matrices. We have utilised the long luminescence lifetime of the probe to measure ONOO− in a time-resolved manner, effectively avoiding the influence of autofluorescence in biological samples. To demonstrate the utility of the Eu(III) probe, we monitored the production of ONOO− in different cell lines, following treatment with a cold atmospheric plasma device commonly used in the clinic for skin wound treatment.

Citation

Breen, C., Pal, R., Elsegood, M. R., Teat, S. J., Iza, F., Wende, K., …Butler, S. J. (2020). Time-resolved luminescence detection of peroxynitrite using a reactivity-based lanthanide probe. Chemical Science, 11(12), 3164-3170. https://doi.org/10.1039/c9sc06053g

Journal Article Type Article
Acceptance Date Feb 14, 2020
Online Publication Date Feb 18, 2020
Publication Date Feb 28, 2020
Deposit Date Apr 15, 2020
Publicly Available Date Apr 15, 2020
Journal Chemical Science
Print ISSN 2041-6520
Electronic ISSN 2041-6539
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 11
Issue 12
Pages 3164-3170
DOI https://doi.org/10.1039/c9sc06053g

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Published Journal Article (Crystal structure data) (692 Kb)
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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/

Copyright Statement
Crystal structure data This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.







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