We use cookies to ensure that we give you the best experience on our website. By continuing to browse this repository, you give consent for essential cookies to be used. You can read more about our Privacy and Cookie Policy.

Durham Research Online
You are in:

Probing the microsolvation environment of the green fluorescent protein chromophore in vacuo.

Zagorec-Marks, Wyatt and Foreman, Madison M. and Verlet, Jan R. R. and Weber, J. Mathias (2020) 'Probing the microsolvation environment of the green fluorescent protein chromophore in vacuo.', Journal of physical chemistry letters., 11 (5). pp. 1940-1946.


We present vibrational and electronic photodissociation spectra of a model chromophore of the green fluorescent protein in complexes with up to two water molecules, prepared in a cryogenic ion trap at 160–180 K. We find the band origin of the singly hydrated chromophore at 20 985 cm–1 (476.5 nm) and observe partially resolved vibrational signatures. While a single water molecule induces only a small shift of the S1 electronic band of the chromophore, without significant change of the Franck–Condon envelope, the spectrum of the dihydrate shows significant broadening and a greater blue shift of the band edge. Comparison of the vibrational spectra with predicted infrared spectra from density functional theory indicates that water molecules can interact with the oxygen atom on the phenolate group or on the imidazole moiety, respectively.

Item Type:Article
Full text:(AM) Accepted Manuscript
Download PDF
Publisher Web site:
Date accepted:19 February 2020
Date deposited:14 May 2020
Date of first online publication:19 February 2020
Date first made open access:19 February 2021

Save or Share this output

Look up in GoogleScholar