Landoni, Beatrice and Viruel, Juan and Gomez, Rocio and Allaby, Robin G. and Brennan, Adrian C. and Pico, F. Xavier and Perez-Barrales, Rocio (2020) 'Microsatellite marker development in the crop wild relative Linum bienne using genome skimming.', Applications in plant sciences., 8 (5). e11349.
Premise: Nuclear microsatellite markers were developed for Linum bienne , the sister species of the crop L. usitatissimum , to provide molecular genetic tools for the investigation of L. bienne genetic diversity and structure. Methods and Results: Fifty microsatellite loci were identified in L. bienne by means of genome skimming, and 44 loci successfully amplified. Of these, 16 loci evenly spread across the L. usitatissimum reference nuclear genome were used for genotyping six L. bienne populations. Excluding one monomorphic locus, the number of alleles per locus ranged from two to 12. Four out of six populations harbored private alleles. The levels of expected and observed heterozygosity were 0.076 to 0.667 and 0.000 to 1.000, respectively. All 16 loci successfully cross‐amplified in L. usitatissimum . Conclusions: The 16 microsatellite loci developed here can be used for population genetic studies in L. bienne , and 28 additional loci that successfully amplified are available for further testing.
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|Publisher Web site:||https://doi.org/10.1002/aps3.11349|
|Publisher statement:||© 2020 Landoni et al. Applications in Plant Sciences is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.|
|Date accepted:||26 January 2020|
|Date deposited:||27 May 2020|
|Date of first online publication:||26 May 2020|
|Date first made open access:||27 May 2020|
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