Maguire, Oliver R. and Zhu, Jiayun and Brittain, William D. G. and Hudson, Alexander S. and Cobb, Steven L. and O’Donoghue, AnnMarie C. (2020) 'N-terminal speciation for native chemical ligation.', Chemical communications., 56 (45). pp. 6114-6117.
Abstract
Native chemical ligation (NCL) enables the chemical synthesis of peptides via reactions between N-terminal thiolates and C-terminal thioesters under mild, aqueous conditions at pH 7–8. Here we demonstrate quantitatively how thiol speciation at N-terminal cysteines and analogues varies significantly depending upon structure at typical pH values used in NCL.
| Item Type: | Article |
|---|---|
| Full text: | (VoR) Version of Record Available under License - Creative Commons Attribution. Download PDF (Supplementary information) (29019Kb) |
| Full text: | (VoR) Version of Record Available under License - Creative Commons Attribution. Download PDF (2159Kb) |
| Status: | Peer-reviewed |
| Publisher Web site: | https://doi.org/10.1039/D0CC01604G |
| Publisher statement: | This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. |
| Date accepted: | 21 April 2020 |
| Date deposited: | 10 June 2020 |
| Date of first online publication: | 23 April 2020 |
| Date first made open access: | 10 June 2020 |
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