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N-terminal speciation for native chemical ligation.

Maguire, Oliver R. and Zhu, Jiayun and Brittain, William D. G. and Hudson, Alexander S. and Cobb, Steven L. and O’Donoghue, AnnMarie C. (2020) 'N-terminal speciation for native chemical ligation.', Chemical communications., 56 (45). pp. 6114-6117.

Abstract

Native chemical ligation (NCL) enables the chemical synthesis of peptides via reactions between N-terminal thiolates and C-terminal thioesters under mild, aqueous conditions at pH 7–8. Here we demonstrate quantitatively how thiol speciation at N-terminal cysteines and analogues varies significantly depending upon structure at typical pH values used in NCL.

Item Type:Article
Full text:(VoR) Version of Record
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Full text:(VoR) Version of Record
Available under License - Creative Commons Attribution.
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Status:Peer-reviewed
Publisher Web site:https://doi.org/10.1039/D0CC01604G
Publisher statement:This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
Date accepted:21 April 2020
Date deposited:10 June 2020
Date of first online publication:23 April 2020
Date first made open access:10 June 2020

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