Solution-state behaviour of algal mono-uronates evaluated by pure shift and compressive sampling NMR techniques

Rowbotham, Jack S.; Aguilar, Juan A.; Kenwright, Alan M.; Greenwell, H. Christopher; Dyer, Philip W.

doi:10.1016/j.carres.2020.108087

Solution-state behaviour of algal mono-uronates evaluated by pure shift and compressive sampling NMR techniques

Rowbotham, Jack S.; Aguilar, Juan A.; Kenwright, Alan M.; Greenwell, H. Christopher; Dyer, Philip W.

Authors

Jack S. Rowbotham

Juan A. Aguilar

Alan M. Kenwright

Professor Chris Greenwell chris.greenwell@durham.ac.uk
Head Of Department

Professor Philip Dyer p.w.dyer@durham.ac.uk
Professor

Abstract

Sodium salts of the algal uronic-acids, d-mannuronic acid (HManA) and l-guluronic acid (HGulA) have been isolated and characterised in solution by nuclear magnetic resonance (NMR) spectroscopy. A suite of recently-described NMR experiments (including pure shift and compressive sampling techniques) were used to provide confident assignments of the pyranose forms of the two uronic acids at various pD values (from 7.5 to 1.4). The resulting high resolution spectra were used to determine several previously unknown parameters for the two acids, including their pKa values, the position of their isomeric equilibria, and their propensity to form furanurono-6,3-lactones. For each of the three parameters, comparisons are drawn with the behaviour of the related D-glucuronic (HGlcA) and D-galacturonic acids (HGalA), which have been previously studied extensively. This paper demonstrates how these new NMR spectroscopic techniques can be applied to better understand the properties of polyuronides and uronide-rich macroalgal biomass.

Citation

Rowbotham, J. S., Aguilar, J. A., Kenwright, A. M., Greenwell, H. C., & Dyer, P. W. (2020). Solution-state behaviour of algal mono-uronates evaluated by pure shift and compressive sampling NMR techniques. Carbohydrate Research, 495, Article 108087. https://doi.org/10.1016/j.carres.2020.108087

Journal Article Type	Article
Acceptance Date	Jun 19, 2020
Online Publication Date	Jul 12, 2020
Publication Date	2020-09
Deposit Date	Jul 13, 2020
Publicly Available Date	Jul 12, 2021
Journal	Carbohydrate Research
Print ISSN	0008-6215
Publisher	Elsevier
Peer Reviewed	Peer Reviewed
Volume	495
Article Number	108087
DOI	https://doi.org/10.1016/j.carres.2020.108087

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© 2020 This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/