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Solution-state behaviour of algal mono-uronates evaluated by pure shift and compressive sampling NMR techniques.

Rowbotham, Jack S. and Aguilar, Juan A. and Kenwright, Alan M. and Greenwell, H. Christopher and Dyer, Philip W. (2020) 'Solution-state behaviour of algal mono-uronates evaluated by pure shift and compressive sampling NMR techniques.', Carbohydrate research., 495 . p. 108087.


Sodium salts of the algal uronic-acids, d-mannuronic acid (HManA) and l-guluronic acid (HGulA) have been isolated and characterised in solution by nuclear magnetic resonance (NMR) spectroscopy. A suite of recently-described NMR experiments (including pure shift and compressive sampling techniques) were used to provide confident assignments of the pyranose forms of the two uronic acids at various pD values (from 7.5 to 1.4). The resulting high resolution spectra were used to determine several previously unknown parameters for the two acids, including their pKa values, the position of their isomeric equilibria, and their propensity to form furanurono-6,3-lactones. For each of the three parameters, comparisons are drawn with the behaviour of the related D-glucuronic (HGlcA) and D-galacturonic acids (HGalA), which have been previously studied extensively. This paper demonstrates how these new NMR spectroscopic techniques can be applied to better understand the properties of polyuronides and uronide-rich macroalgal biomass.

Item Type:Article
Full text:Publisher-imposed embargo until 12 July 2021.
(AM) Accepted Manuscript
Available under License - Creative Commons Attribution Non-commercial No Derivatives.
File format - PDF
Publisher Web site:
Publisher statement:© 2020 This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Date accepted:19 June 2020
Date deposited:13 July 2020
Date of first online publication:12 July 2020
Date first made open access:12 July 2021

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