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An expression atlas of variant ionotropic glutamate receptors identifies a molecular basis of carbonation sensing

Sánchez-Alcañiz, J.A.; Silbering, A.F.; Croset, V.; Zappia, G.; Sivasubramaniam, A.K.; Abuin, L.; Sahai, S.Y.; Münch, D.; Steck, K.; Auer, T.O.; Cruchet, S.; Neagu-Maier, G.L.; Sprecher, S.G.; Ribeiro, C.; Yapici, N.; Benton, R.

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Authors

J.A. Sánchez-Alcañiz

A.F. Silbering

G. Zappia

A.K. Sivasubramaniam

L. Abuin

S.Y. Sahai

D. Münch

K. Steck

T.O. Auer

S. Cruchet

G.L. Neagu-Maier

S.G. Sprecher

C. Ribeiro

N. Yapici

R. Benton



Abstract

Through analysis of the Drosophila ionotropic receptors (IRs), a family of variant ionotropic glutamate receptors, we reveal that most IRs are expressed in peripheral neuron populations in diverse gustatory organs in larvae and adults. We characterise IR56d, which defines two anatomically-distinct neuron classes in the proboscis: one responds to carbonated solutions and fatty acids while the other represents a subset of sugar- and fatty acid-sensing cells. Mutational analysis indicates that IR56d, together with the broadly-expressed co-receptors IR25a and IR76b, is essential for physiological responses to carbonation and fatty acids, but not sugars. We further demonstrate that carbonation and fatty acids both promote IR56d-dependent attraction of flies, but through different behavioural outputs. Our work provides a toolkit for investigating taste functions of IRs, defines a subset of these receptors required for carbonation sensing, and illustrates how the gustatory system uses combinatorial expression of sensory molecules in distinct neurons to coordinate behaviour.

Citation

Sánchez-Alcañiz, J., Silbering, A., Croset, V., Zappia, G., Sivasubramaniam, A., Abuin, L., …Benton, R. (2018). An expression atlas of variant ionotropic glutamate receptors identifies a molecular basis of carbonation sensing. Nature Communications, 9(1), Article 4252. https://doi.org/10.1038/s41467-018-06453-1

Journal Article Type Article
Acceptance Date Sep 6, 2018
Online Publication Date Oct 12, 2018
Publication Date 2018
Deposit Date Jul 27, 2020
Publicly Available Date Aug 26, 2020
Journal Nature Communications
Publisher Nature Research
Peer Reviewed Peer Reviewed
Volume 9
Issue 1
Article Number 4252
DOI https://doi.org/10.1038/s41467-018-06453-1

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http://creativecommons.org/licenses/by/4.0/

Copyright Statement
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.




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