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The application of temperature sensitivity CRISPR/LbCpf1 (LbCas12a) mediated genome editing in allotetraploid cotton (G. hirsutum) and creation of nontransgenic, gossypol‐free cotton

Li, B.; Liang, S.; Alariqi, M.; Wang, F.; Wang, G.; Wang, Q.; Xu, Z.; Yu, L.; Zafar, M.N.; Sun, L.; Si, H.; Yuan, D.; Guo, W.; Wang, Y.; Lindsey, K.; Zhang, X.; Jin, S.

The application of temperature sensitivity CRISPR/LbCpf1 (LbCas12a) mediated genome editing in allotetraploid cotton (G. hirsutum) and creation of nontransgenic, gossypol‐free cotton Thumbnail


Authors

B. Li

S. Liang

M. Alariqi

F. Wang

G. Wang

Q. Wang

Z. Xu

L. Yu

M.N. Zafar

L. Sun

H. Si

D. Yuan

W. Guo

Y. Wang

X. Zhang

S. Jin



Abstract

Cotton (Gossypium hirsutum) is an allotetraploid species and a typical thermophilic crop that can survive and grow well under temperatures up to 45°C. CRISPR/LbCpf1 (LbCas12a) is a temperature‐sensitive system for plant genome editing (Malzahn et al., 2019) and has been successfully applied in species such as rice, soybean, tobacco, maize and cotton (Lee et al., 2019; Li et al., 2018; Tang et al., 2017; Xu et al., 2019). However, the temperature sensitivity of LbCpf1 has not been tested in cotton yet, a high temperature‐resistant crop. In order to improve LbCpf1 efficiency and determine the optimum temperature for cotton genome editing, we investigated the effects of different temperatures on LbCpf1 activity and genome editing efficiency. Moreover, we created nontransgenic and glandless cotton plants with seeds free of gossypol, representing a valuable germplasm resource for cotton breeding.

Citation

Li, B., Liang, S., Alariqi, M., Wang, F., Wang, G., Wang, Q., …Jin, S. (2020). The application of temperature sensitivity CRISPR/LbCpf1 (LbCas12a) mediated genome editing in allotetraploid cotton (G. hirsutum) and creation of nontransgenic, gossypol‐free cotton. Plant Biotechnology Journal, 19(2), 221-223. https://doi.org/10.1111/pbi.13470

Journal Article Type Article
Acceptance Date Aug 11, 2020
Online Publication Date Sep 14, 2020
Publication Date 2020-02
Deposit Date Sep 1, 2020
Publicly Available Date Mar 29, 2024
Journal Plant Biotechnology Journal
Print ISSN 1467-7644
Electronic ISSN 1467-7652
Publisher Association of Applied Biologists
Peer Reviewed Peer Reviewed
Volume 19
Issue 2
Pages 221-223
DOI https://doi.org/10.1111/pbi.13470

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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/

Copyright Statement
Advance online version © 2020 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.





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