Lynsay I. Blake
An Unexpectedly Broad Thermal and Salinity-Tolerant Estuarine Methanogen Community
Blake, Lynsay I.; Sherry, Angela; Mejeha, Obioma K.; Leary, Peter; Coombs, Henry; Stone, Wendy; Head, Ian M.; Gray, Neil D.
Authors
Angela Sherry
Obioma K. Mejeha
Peter Leary
Henry Coombs
Wendy Stone
Ian M. Head
Neil D. Gray
Abstract
Moderately thermophilic (Tmax, ~55 °C) methanogens are identified after extended enrichments from temperate, tropical and low-temperature environments. However, thermophilic methanogens with higher growth temperatures (Topt ≥ 60 °C) are only reported from high-temperature environments. A microcosm-based approach was used to measure the rate of methane production and methanogen community structure over a range of temperatures and salinities in sediment from a temperate estuary. We report short-term incubations (<48 h) revealing methanogens with optimal activity reaching 70 °C in a temperate estuary sediment (in situ temperature 4–5 °C). While 30 °C enrichments amended with acetate, H2 or methanol selected for corresponding mesophilic trophic groups, at 60 °C, only hydrogenotrophs (genus Methanothermobacter) were observed. Since these methanogens are not known to be active under in situ temperatures, we conclude constant dispersal from high temperature habitats. The likely provenance of the thermophilic methanogens was studied by enrichments covering a range of temperatures and salinities. These enrichments indicated that the estuarine sediment hosted methanogens encompassing the global activity envelope of most cultured species. We suggest that estuaries are fascinating sink and source environments for microbial function study.
Citation
Blake, L. I., Sherry, A., Mejeha, O. K., Leary, P., Coombs, H., Stone, W., …Gray, N. D. (2020). An Unexpectedly Broad Thermal and Salinity-Tolerant Estuarine Methanogen Community. Microorganisms, 8(10), Article 1467. https://doi.org/10.3390/microorganisms8101467
Journal Article Type | Article |
---|---|
Acceptance Date | Sep 22, 2020 |
Online Publication Date | Sep 24, 2020 |
Publication Date | 2020-10 |
Deposit Date | Oct 6, 2020 |
Publicly Available Date | Oct 6, 2020 |
Journal | Microorganisms |
Publisher | MDPI |
Peer Reviewed | Peer Reviewed |
Volume | 8 |
Issue | 10 |
Article Number | 1467 |
DOI | https://doi.org/10.3390/microorganisms8101467 |
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Copyright Statement
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution
(CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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