Cookies

We use cookies to ensure that we give you the best experience on our website. By continuing to browse this repository, you give consent for essential cookies to be used. You can read more about our Privacy and Cookie Policy.


Durham Research Online
You are in:

Crystal structure of the GDP-bound GTPase domain of Rab5a from Leishmania donovani.

Zohib, Muhammad and Maheshwari, Diva and Pal, Ravi Kant and Freitag-Pohl, Stefanie and Biswal, Bichitra Kumar and Pohl, Ehmke and Arora, Ashish (2020) 'Crystal structure of the GDP-bound GTPase domain of Rab5a from Leishmania donovani.', Acta crystallographica section F : structural biology communications., 76 (11). pp. 544-556.

Abstract

Eukaryotic Rab5s are highly conserved small GTPase-family proteins that are involved in the regulation of early endocytosis. Leishmania donovani Rab5a regulates the sorting of early endosomes that are involved in the uptake of essential nutrients through fluid-phase endocytosis. Here, the 1.80 Å resolution crystal structure of the N-terminal GTPase domain of L. donovani Rab5a in complex with GDP is presented. The crystal structure determination was enabled by the design of specific single-site mutations and two deletions that were made to stabilize the protein for previous NMR studies. The structure of LdRab5a shows the canonical GTPase fold, with a six-stranded central mixed β-sheet surrounded by five α-helices. The positions of the Switch I and Switch II loops confirm an open conformation, as expected in the absence of the γ-phosphate. However, in comparison to other GTP-bound and GDP-bound homologous proteins, the Switch I region traces a unique disposition in LdRab5a. One magnesium ion is bound to the protein at the GTP-binding site. Molecular-dynamics simulations indicate that the GDP-bound structure exhibits higher stability than the apo structure. The GDP-bound LdRab5a structure presented here will aid in efforts to unravel its interactions with its regulators, including the guanine nucleotide-exchange factor, and will lay the foundation for a structure-based search for specific inhibitors

Item Type:Article
Full text:(AM) Accepted Manuscript
Download PDF (Electronic reprint)
(1877Kb)
Status:Peer-reviewed
Publisher Web site:https://doi.org/10.1107/S2053230X20013722
Date accepted:13 October 2020
Date deposited:19 November 2020
Date of first online publication:November 2020
Date first made open access:19 November 2020

Save or Share this output

Export:
Export
Look up in GoogleScholar