17O NMR spectroscopy as a tool to study hydrogen bonding of cholesterol in lipid bilayers

Rowlands, Lucy J.; Marks, Adam; Sanderson, John M.; Law, Robert V.

doi:10.1039/d0cc05466f

17O NMR spectroscopy as a tool to study hydrogen bonding of cholesterol in lipid bilayers

Rowlands, Lucy J.; Marks, Adam; Sanderson, John M.; Law, Robert V.

Authors

Lucy J. Rowlands

Adam Marks

Dr John Sanderson j.m.sanderson@durham.ac.uk
Associate Professor

Robert V. Law

Abstract

Cholesterol is a crucial component of biological membranes and can interact with other membrane components through hydrogen bonding. NMR spectroscopy has been used previously to investigate this bonding, however this study represents the first 17O NMR spectroscopy study of isotopically enriched cholesterol. We demonstrate the 17O chemical shift is dependent on hydrogen bonding, providing a novel method for the study of cholesterol in bilayers.

Citation

Rowlands, L. J., Marks, A., Sanderson, J. M., & Law, R. V. (2020). 17O NMR spectroscopy as a tool to study hydrogen bonding of cholesterol in lipid bilayers. Chemical Communications, 56(92), 14499-14502. https://doi.org/10.1039/d0cc05466f

Journal Article Type	Article
Acceptance Date	Oct 28, 2020
Online Publication Date	Nov 5, 2020
Publication Date	Nov 28, 2020
Deposit Date	Nov 5, 2020
Publicly Available Date	Feb 8, 2021
Journal	Chemical Communications
Print ISSN	1359-7345
Electronic ISSN	1364-548X
Publisher	Royal Society of Chemistry
Peer Reviewed	Peer Reviewed
Volume	56
Issue	92
Pages	14499-14502
DOI	https://doi.org/10.1039/d0cc05466f