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Isolation and characterisation of bacteriophages with activity against invasive non-typhoidal Salmonella causing blood-stream infection in Malawi

Rodwell, E.V.; Wenner, N.; Pulford, C.V.; Cai, Y.; Bowers-Barnard, A.; Beckett, A.; Rigby, J.; Picton, D.M.; Blower, T.R.; Feasey, N.A.; Hinton, J.C.D.; Perez-Sepulveda, B.M.

Isolation and characterisation of bacteriophages with activity against invasive non-typhoidal Salmonella causing blood-stream infection in Malawi Thumbnail


Authors

E.V. Rodwell

N. Wenner

C.V. Pulford

Y. Cai

A. Bowers-Barnard

A. Beckett

J. Rigby

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David Picton david.m.picton@durham.ac.uk
PGR Student Doctor of Philosophy

N.A. Feasey

J.C.D. Hinton

B.M. Perez-Sepulveda



Abstract

In recent years, novel lineages of invasive non-typhoidal Salmonella (iNTS) serovars Typhimurium and Enteritidis have been identified in patients with bloodstream infection in sub-Saharan Africa. Here, we isolated and characterised 32 phages capable of infecting S. Typhimurium and S. Enteritidis, from water sources in Malawi and the UK. The phages were classified in three major phylogenetic clusters that were geographically distributed. In terms of host range, Cluster 1 phages were able to infect all bacterial hosts tested, whereas Clusters 2 and 3 had a more restricted profile. Cluster 3 contained two sub-clusters, and 3.b contained the most novel isolates. This study represents the first exploration of the potential for phages to target the lineages of Salmonella that are responsible for bloodstream infections in sub-Saharan Africa.

Citation

Rodwell, E., Wenner, N., Pulford, C., Cai, Y., Bowers-Barnard, A., Beckett, A., …Perez-Sepulveda, B. (2021). Isolation and characterisation of bacteriophages with activity against invasive non-typhoidal Salmonella causing blood-stream infection in Malawi. Viruses, 13(3), Article 478. https://doi.org/10.3390/v13030478

Journal Article Type Article
Acceptance Date Mar 11, 2021
Online Publication Date Mar 15, 2021
Publication Date 2021-03
Deposit Date Mar 11, 2021
Publicly Available Date Mar 18, 2021
Journal Viruses
Publisher MDPI
Peer Reviewed Peer Reviewed
Volume 13
Issue 3
Article Number 478
DOI https://doi.org/10.3390/v13030478
Related Public URLs https://doi.org/10.1101/2021.02.18.431836

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Copyright Statement
© 2021 by the authors.
Licensee MDPI, Basel, Switzerland.
This article is an open access article
distributed under the terms and
conditions of the Creative Commons
Attribution (CC BY) license (https://
creativecommons.org/licenses/by/
4.0/)





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