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LightBox: a multiwell plate illumination system for photoactive molecule characterization

Bounds, A.D.; Bailey, R.; Adams, C.T.; Callaghan, D.C.; Girkin, J.M.

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Authors

R. Bailey

C.T. Adams

D.C. Callaghan



Abstract

Multiwell plates (MWPs) are the workhorses of the life sciences. However, biophotonics research with MWPs is limited, in part due to the lack of equipment suitable for photo‐irradiation of photoactive molecules in a MWP‐suitable, high‐throughput manner, either commercially or through open‐source MWP systems. Here we present “LightBox”, a calibrated controllable MWP illumination system with broad applications including screening of photoactive molecules and characterization of photocatalytic chemicals. LightBox is a high intensity, accurately controllable, uniform illumination system designed for MWPs with electronics and a control unit that provides a simple and intuitive interface. LightBox can reach intensities of 0.23 mW/mm2 at wavelengths of 405 nm with variance between well sites of <5%. The usefulness of LightBox is demonstrated by assessing the IC50 of a photosensitizing compound using a live/dead assay following simultaneous irradiation of the sample at a range of concentrations, eliminating uncontrolled variables between concentrations and drastically increasing assessment speed.

Citation

Bounds, A., Bailey, R., Adams, C., Callaghan, D., & Girkin, J. (2021). LightBox: a multiwell plate illumination system for photoactive molecule characterization. Journal of Biophotonics, 14(5), Article e202000481. https://doi.org/10.1002/jbio.202000481

Journal Article Type Article
Acceptance Date Jan 28, 2021
Online Publication Date Feb 12, 2021
Publication Date 2021-05
Deposit Date Dec 2, 2020
Publicly Available Date Mar 28, 2024
Journal Journal of Biophotonics
Print ISSN 1864-063X
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 14
Issue 5
Article Number e202000481
DOI https://doi.org/10.1002/jbio.202000481

Files

Published Journal Article (Early View) (1.4 Mb)
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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/

Copyright Statement
Early View © 2021 The Authors. Journal of Biophotonics published by Wiley‐VCH GmbH.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.






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