Cookies

We use cookies to ensure that we give you the best experience on our website. By continuing to browse this repository, you give consent for essential cookies to be used. You can read more about our Privacy and Cookie Policy.


Durham Research Online
You are in:

LightBox: a multiwell plate illumination system for photoactive molecule characterization

Bounds, A. D. and Bailey, R. and Adams, C. T. and Callaghan, D. C. and Girkin, J. M. (2021) 'LightBox: a multiwell plate illumination system for photoactive molecule characterization.', Journal of Biophotonics, 14 (5). e202000481.

Abstract

Multiwell plates (MWPs) are the workhorses of the life sciences. However, biophotonics research with MWPs is limited, in part due to the lack of equipment suitable for photo‐irradiation of photoactive molecules in a MWP‐suitable, high‐throughput manner, either commercially or through open‐source MWP systems. Here we present “LightBox”, a calibrated controllable MWP illumination system with broad applications including screening of photoactive molecules and characterization of photocatalytic chemicals. LightBox is a high intensity, accurately controllable, uniform illumination system designed for MWPs with electronics and a control unit that provides a simple and intuitive interface. LightBox can reach intensities of 0.23 mW/mm2 at wavelengths of 405 nm with variance between well sites of <5%. The usefulness of LightBox is demonstrated by assessing the IC50 of a photosensitizing compound using a live/dead assay following simultaneous irradiation of the sample at a range of concentrations, eliminating uncontrolled variables between concentrations and drastically increasing assessment speed.

Item Type:Article
Full text:(VoR) Version of Record
Available under License - Creative Commons Attribution 4.0.
Download PDF (Early View)
(1403Kb)
Full text:(VoR) Version of Record
Available under License - Creative Commons Attribution 4.0.
Download PDF
(1403Kb)
Status:Peer-reviewed
Publisher Web site:https://doi.org/10.1002/jbio.202000481
Publisher statement:© 2021 The Authors. Journal of Biophotonics published by Wiley‐VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Date accepted:28 January 2021
Date deposited:07 April 2021
Date of first online publication:12 February 2021
Date first made open access:07 April 2021

Save or Share this output

Export:
Export
Look up in GoogleScholar