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NETWORKED2‐Subfamily Proteins Regulate the Cortical Actin Cytoskeleton of Growing Pollen Tubes and Polarised Pollen Tube Growth

Duckney, Patrick and Kroon, Johan T. and Dixon, Martin R. and Hawkins, Timothy J. and Deeks, Michael J. and Hussey, Patrick J. (2021) 'NETWORKED2‐Subfamily Proteins Regulate the Cortical Actin Cytoskeleton of Growing Pollen Tubes and Polarised Pollen Tube Growth.', New Phytologist, 231 (1). pp. 152-164.


We have recently characterised NET2A as a pollen‐specific actin‐binding protein which binds F‐actin at the plasma membrane of growing pollen tubes. However, the role of NET2 proteins in pollen development and fertilisation have yet to be elucidated. To further characterise the role of Arabidopsis NET2 proteins in pollen development and fertilisation, we analysed the subcellular localisation of NET2A over the course of pollen grain development, and investigated the role of the NET2 family using net2 loss‐of‐function mutants. We observed NET2A to localise to the F‐actin cytoskeleton in developing pollen grains as it underwent striking structural reorganisations at specific stages of development and during germination, and pollen tube growth. Furthermore, net2 loss‐of‐function mutants exhibited striking morphological defects in the early stages of pollen tube growth, arising from frequent alterations to pollen tube growth trajectory. We observed defects in the cortical actin cytoskeleton and actin‐driven subcellular processes in net2 mutant pollen tubes. We demonstrate that NET2 proteins are essential for normal actin‐driven pollen development highlighting an important role for the NET2 family members in regulating pollen tube growth during fertilisation.

Item Type:Article
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Publisher statement:© 2021 The Authors New Phytologist © 2021 New Phytologist Foundation This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Date accepted:29 March 2021
Date deposited:21 April 2021
Date of first online publication:16 April 2021
Date first made open access:17 June 2021

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