B. Usher
Crystal structure of the anti-CRISPR repressor Aca2
Usher, B.; Birkholz, N.; Beck, I.N.; Fagerlund, R.D.; Jackson, S.A.; Fineran, P.C.; Blower, T.R.
Authors
N. Birkholz
I.N. Beck
R.D. Fagerlund
S.A. Jackson
P.C. Fineran
Professor Tim Blower timothy.blower@durham.ac.uk
Professor
Abstract
Bacteria use adaptive CRISPR-Cas immune mechanisms to protect from invasion by bacteriophages and other mobile genetic elements. In response, bacteriophages and mobile genetic elements have co-evolved anti-CRISPR proteins to inhibit the bacterial defense. We and others have previously shown that anti-CRISPR associated (Aca) proteins can regulate this anti-CRISPR counter-attack. Here, we report the first structure of an Aca protein, the Aca2 DNA-binding transcriptional autorepressor from Pectobacterium carotovorum bacteriophage ZF40, determined to 1.34 Å. Aca2 presents a conserved N-terminal helix-turn-helix DNA-binding domain and a previously uncharacterized C-terminal dimerization domain. Dimerization positions the Aca2 recognition helices for insertion into the major grooves of target DNA, supporting its role in regulating anti-CRISPRs. Furthermore, database comparisons identified uncharacterized Aca2 structural homologs in pathogenic bacteria, suggesting that Aca2 represents the first characterized member of a more widespread family of transcriptional regulators.
Citation
Usher, B., Birkholz, N., Beck, I., Fagerlund, R., Jackson, S., Fineran, P., & Blower, T. (2021). Crystal structure of the anti-CRISPR repressor Aca2. Journal of Structural Biology, 213(3), Article 107752. https://doi.org/10.1016/j.jsb.2021.107752
Journal Article Type | Article |
---|---|
Acceptance Date | Jun 6, 2021 |
Online Publication Date | Jun 8, 2021 |
Publication Date | 2021-09 |
Deposit Date | Jun 7, 2021 |
Publicly Available Date | Mar 28, 2024 |
Journal | Journal of Structural Biology |
Print ISSN | 1047-8477 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 213 |
Issue | 3 |
Article Number | 107752 |
DOI | https://doi.org/10.1016/j.jsb.2021.107752 |
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Copyright Statement
© 2021 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)
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