Targeted pH switched europium complexes monitoring receptor internalisation in living cells

Fradgley, Jack D.; Starck, Matthieu; Laget, Michel; Bourrier, Emmanuel; Dupuis, Elodie; Lamarque, Laurent; Trinquet, Eric; Zwier, Jurriaan M.; Parker, David

doi:10.1039/d1cc01029h

Targeted pH switched europium complexes monitoring receptor internalisation in living cells

Fradgley, Jack D.; Starck, Matthieu; Laget, Michel; Bourrier, Emmanuel; Dupuis, Elodie; Lamarque, Laurent; Trinquet, Eric; Zwier, Jurriaan M.; Parker, David

Authors

Jack D. Fradgley

Matthieu Starck

Michel Laget

Emmanuel Bourrier

Elodie Dupuis

Laurent Lamarque

Eric Trinquet

Jurriaan M. Zwier

Professor David Parker david.parker@durham.ac.uk
Emeritus Professor

Abstract

We report the design and evaluation of pH responsive luminescent europium(III) probes that allow conjugation to targeting vectors to monitor receptor internalisation in cells. The approach adopted here can be used to tag proteins selectively and to monitor uptake into more acidic organelles, thereby enhancing the performance of time-resolved internalisation assays that require pH monitoring in real time.

Citation

Fradgley, J. D., Starck, M., Laget, M., Bourrier, E., Dupuis, E., Lamarque, L., …Parker, D. (2021). Targeted pH switched europium complexes monitoring receptor internalisation in living cells. Chemical Communications, 57(47), 5814-5817. https://doi.org/10.1039/d1cc01029h

Journal Article Type	Article
Acceptance Date	May 4, 2021
Online Publication Date	May 4, 2021
Publication Date	2021
Deposit Date	Aug 25, 2021
Publicly Available Date	Aug 25, 2021
Journal	Chemical Communications
Print ISSN	1359-7345
Electronic ISSN	1364-548X
Publisher	Royal Society of Chemistry
Peer Reviewed	Peer Reviewed
Volume	57
Issue	47
Pages	5814-5817
DOI	https://doi.org/10.1039/d1cc01029h