Regulation of prokaryotic adenylyl cyclases by CO₂

Hammer, A.; Hodgson, D.R.W.; Cann, M.J.

doi:10.1042/bj20060372

Regulation of prokaryotic adenylyl cyclases by CO₂

Hammer, A.; Hodgson, D.R.W.; Cann, M.J.

Authors

A. Hammer

Professor David Hodgson d.r.w.hodgson@durham.ac.uk
Professor

Professor Martin Cann m.j.cann@durham.ac.uk
Professor

Abstract

The Slr1991 adenylyl cyclase of the model prokaroyte Synechocystis PCC 6803 was stimulated 2-fold at 20 mM total Ci (inorganic carbon) atpH 7.5 through an increase in kcat.Adose response demonstrated an EC50 of 52.7 mMtotal Ci at pH 6.5. Slr1991 adenylyl cyclase was activated by CO2, but not by HCO3−. CO2 regulation of adenylyl cyclase was conserved in the CyaB1 adenylyl cyclase of Anabaena PCC 7120. These adenylyl cyclases represent the only identified signalling enzymes directly activated by CO2. The findings prompt an urgent reassessment of the activating carbon species for proposed HCO3−-activated adenylyl cyclases.

Citation

Hammer, A., Hodgson, D., & Cann, M. (2006). Regulation of prokaryotic adenylyl cyclases by CO₂. Biochemical Journal, 396(2), 215-218. https://doi.org/10.1042/bj20060372

Journal Article Type	Article
Publication Date	Jan 1, 2006
Deposit Date	May 10, 2007
Publicly Available Date	Nov 27, 2014
Journal	Biochemical Journal
Print ISSN	0264-6021
Publisher	Portland Press
Peer Reviewed	Peer Reviewed
Volume	396
Issue	2
Pages	215-218
DOI	https://doi.org/10.1042/bj20060372
Keywords	Adenylyl cyclase (adenyl cyclase, Adenylate cyclase), Bicarbonate (HCO3−), cAMP, Carbon dioxide (CO2), Synechocystis.

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Copyright Statement
This research was originally published in Biochemical journal. Hammer, A., Hodgson, D. R. W., Cann, M.J., Regulation of prokaryotic adenylyl cyclases by CO2. Biochemical journal. 2006; 396: 215-218 © the Biochemical Society