Peptide Lipidation in Lysophospholipid Micelles and Lysophospholipid-Enriched Membranes

Esmaeil, Vian S; Britt, Hannah M; Mosely, Jackie A; Sanderson, John M

doi:10.1039/d1fd00030f

Peptide Lipidation in Lysophospholipid Micelles and Lysophospholipid-Enriched Membranes

Esmaeil, Vian S; Britt, Hannah M; Mosely, Jackie A; Sanderson, John M

Authors

Vian S Esmaeil

Hannah M Britt

Jackie A Mosely

Dr John Sanderson j.m.sanderson@durham.ac.uk
Associate Professor

Abstract

Acyl transfer from lipids to membrane-associated peptides is a well-documented process, leading to the generation of a lipidated peptide and a lysolipid. In this article, we demonstrate that acyl transfer from lysophosphatidylcholines (lysoPCs) to the peptide melittin also occurs, both in micelles of pure lysolipid and in lipid/lysolipid mixtures. In the case of bilayers containing lysolipids, acyl transfer from the lysolipid is marginally favoured over transfer from the lipid. In pure bilayers of saturated lipids, the introduction of even small amounts of lysolipid appears to significantly increase the reactivity towards lipidation.

Citation

Esmaeil, V. S., Britt, H. M., Mosely, J. A., & Sanderson, J. M. (2021). Peptide Lipidation in Lysophospholipid Micelles and Lysophospholipid-Enriched Membranes. Faraday Discussions, 2021(232), 282-294. https://doi.org/10.1039/d1fd00030f

Journal Article Type	Article
Acceptance Date	Jul 16, 2021
Online Publication Date	Jul 29, 2021
Publication Date	Dec 1, 2021
Deposit Date	Jul 27, 2021
Publicly Available Date	Oct 7, 2021
Journal	Faraday Discussions
Print ISSN	1359-6640
Electronic ISSN	1364-5498
Publisher	Royal Society of Chemistry
Peer Reviewed	Peer Reviewed
Volume	2021
Issue	232
Pages	282-294
DOI	https://doi.org/10.1039/d1fd00030f