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Applying TADF Emitters in Bioimaging and Sensing—A Novel Approach Using Liposomes for Encapsulation and Cellular Uptake

Smith, Poppy O.; Black, Dominic J.; Pal, Robert; Avó, João; Dias, Fernando B.; Linthwaite, Victoria L.; Cann, Martin J.; Pålsson, Lars-Olof

Applying TADF Emitters in Bioimaging and Sensing—A Novel Approach Using Liposomes for Encapsulation and Cellular Uptake Thumbnail


Authors

Poppy O. Smith

Dominic J. Black

João Avó

Victoria L. Linthwaite



Abstract

A new method for facilitating the delivery, uptake and intracellular localisation of thermally activated delayed fluorescence (TADF) complexes was developed. First, confinement of TADF complexes in liposomes was demonstrated, which were subsequently used as the delivery vehicle for cellular uptake. Confocal fluorescence microscopy showed TADF complexes subsequently localise in the cytoplasm of HepG2 cells. The procedures developed in this work included the removal of molecular oxygen in the liposome preparation without disrupting the liposome structures. Time-resolved fluorescence microscopy (point scanning) showed initial prompt fluorescence followed by a weak, but detectable, delayed fluorescence component for liposomal TADF internalised in HepG2 cells. By demonstrating that it is possible to deliver un-functionalised and/or unshielded TADF complexes, a sensing function for TADFs, such as molecular oxygen, can be envisaged.

Citation

Smith, P. O., Black, D. J., Pal, R., Avó, J., Dias, F. B., Linthwaite, V. L., …Pålsson, L. (2021). Applying TADF Emitters in Bioimaging and Sensing—A Novel Approach Using Liposomes for Encapsulation and Cellular Uptake. Frontiers in Chemistry, 9, Article 743928. https://doi.org/10.3389/fchem.2021.743928

Journal Article Type Article
Acceptance Date Aug 16, 2021
Online Publication Date Sep 1, 2021
Publication Date 2021
Deposit Date Sep 8, 2021
Publicly Available Date Mar 29, 2024
Journal Frontiers in Chemistry
Publisher Frontiers Media
Peer Reviewed Peer Reviewed
Volume 9
Article Number 743928
DOI https://doi.org/10.3389/fchem.2021.743928

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Published Journal Article (1.4 Mb)
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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/

Copyright Statement
© 2021 Smith, Black, Pal, Avó, Dias, Linthwaite, Cann and Pålsson. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.





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