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Biophysical insights from supported lipid patches

Miller, Ethan; Stubbington, Liam; Dinet, Celine; Staykova, Margarita

Authors

Ethan Miller

Liam Stubbington

Celine Dinet



Contributors

Aleš Iglič
Editor

Michael Rappolt
Editor

Ana J. García-Sáez
Editor

Abstract

Supported lipid bilayer (SLB) patches have emerged in recent years as a convenient system to study membrane-substrate interactions, membrane mechanics, and interaction of membranes with other molecules, due to the possibility of tracking their area changes in response to various stimuli. In this paper, we present protocols for the formation of SLB patches on glass and polydimethylsiloxane substrates via vesicle fusion, describe the interaction forces between the lipid membrane and the substrate, and discuss the force dependence on the buffer composition, membrane lipid species, the surface properties of the substrate. Following that, we present own and others results that utilize SLB patches to study 1) membrane sorption of cholesterol, peptides and other surface active molecules, 2) the effects of the substrate on lipid phase separation, and 3) the mechanics of substrate-adhered lipid membranes and their biophysical implications. Finally we discuss methods to obtain SLB patches of more complex composition and geometry.

Citation

Miller, E., Stubbington, L., Dinet, C., & Staykova, M. (2019). Biophysical insights from supported lipid patches. In A. Iglič, M. Rappolt, & A. J. García-Sáez (Eds.), Advances in Biomembranes and Lipid Self-Assembly (23-48). Elsevier. https://doi.org/10.1016/bs.abl.2019.01.004

Acceptance Date Jan 16, 2019
Online Publication Date Feb 20, 2019
Publication Date 2019
Deposit Date Mar 27, 2019
Publisher Elsevier
Pages 23-48
Series Number 29
Book Title Advances in Biomembranes and Lipid Self-Assembly.
DOI https://doi.org/10.1016/bs.abl.2019.01.004


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