Casson, S. and Spencer, M. and Walker, K. and Lindsey, K. (2005) 'Laser capture microdissection for the analysis of gene expression during embryogenesis of Arabidopsis.', Plant journal., 42 (1). pp. 111-123.
It is during embryogenesis that the body plan of the developing plant is established. Analysis of gene expression during embryogenesis has been limited due to the technical difficulty of accessing the developing embryo. Here we demonstrate that laser capture microdissection can be applied to the analysis of embryogenesis. We show how this technique can be used in concert with DNA microarray for the large-scale analysis of gene expression in apical and basal domains of the globular-stage and heart-stage embryo, respectively, when critical events of polarity, symmetry and biochemical differentiation are established. This high resolution spatial analysis shows that up to approximately 65% of the genome is expressed in the developing embryo, and that differential expression of a number of gene classes can be detected. We discuss the validity of this approach for the functional analysis of both published and previously uncharacterized essential genes.
|Full text:||Full text not available from this repository.|
|Publisher Web site:||http://dx.doi.org/10.1111/j.1365-313X.2005.02355.x|
|Date accepted:||No date available|
|Date deposited:||No date available|
|Date of first online publication:||April 2005|
|Date first made open access:||No date available|
Save or Share this output
|Look up in GoogleScholar|