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Reversion of fruit‐dependent inhibition of flowering in Citrus requires sprouting of buds with epigenetically silenced CcMADS19

Mesejo, Carlos and Marzal, Andres and Martinez Fuentes, Amparo and Reig, Carmina and de Lucas, Miguel and Iglesias, Domingo J. and Primo Millo, Eduardo and Blazquez, Miguel A. and Agusti, Manuel (2022) 'Reversion of fruit‐dependent inhibition of flowering in Citrus requires sprouting of buds with epigenetically silenced CcMADS19.', New Phytologist, 233 (1). 526 - 533.


In Citrus, the response to environmental floral inductive signals is inhibited by the presence of developing fruits. The mechanism involves epigenetic activation of the CcMADS19 locus (FLC orthologue), encoding a floral repressor. To understand how this epigenetic regulation is reverted to allow flowering in the following season, we have forced precocious sprouting of axillary buds in fruit-bearing shoots, and examined the competence to floral inductive signals of old and new leaves derived from them. We have found that CcMADS19 is enriched in repressive H3K27me3 marks in young, but not old leaves, revealing that axillary buds retain a silenced version of the floral repressor that is mitotically transmitted to the newly emerging leaves, which are able to induce flowering. Therefore, we propose that flowering in Citrus is necessarily preceded by vegetative sprouting, so that the competence to respond to floral inductive signals is reset in the new leaves.

Item Type:Article
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Publisher statement:This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
Date accepted:10 August 2021
Date deposited:02 December 2021
Date of first online publication:02 December 2021
Date first made open access:02 December 2021

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