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The nucleoporin Nup153 is required for nuclear pore basket formation, nuclear pore complex anchoring and import of a subset of nuclear proteins

Walther, TC; Fornerod, M; Pickersgill, H; Goldberg, M; Allen, TD; Mattaj, IW.

Authors

TC Walther

M Fornerod

H Pickersgill

TD Allen

IW. Mattaj



Abstract

The nuclear pore complex (NPC) is a large proteinaceous structure through which bidirectional transport of macromolecules across the nuclear envelope (NE) takes place. Nup153 is a peripheral NPC component that has been implicated in protein and RNP transport and in the interaction of NPCs with the nuclear lamina. Here, Nup153 is localized by immunogold electron microscopy to a position on the nuclear ring of the NPC. Nuclear reconstitution is used to investigate the role of Nup153 in nucleo- cytoplasmic transport and NPC architecture. NPCs assembled in the absence of Nup153 lacked several nuclear basket components, were unevenly distributed in the NE and, unlike wild-type NPCs, were mobile within the NE. Importin /-mediated protein import into the nucleus was strongly reduced in the absence of Nup153, while transportin-mediated import was unaffected. This was due to a reduction in import complex translocation rather than to defective receptor recycling. Our results therefore reveal functions for Nup153 in NPC assembly, in anchoring NPCs within the NE and in mediating specific nuclear import events.

Citation

Walther, T., Fornerod, M., Pickersgill, H., Goldberg, M., Allen, T., & Mattaj, I. (2001). The nucleoporin Nup153 is required for nuclear pore basket formation, nuclear pore complex anchoring and import of a subset of nuclear proteins. The EMBO Journal, 20(20), 5703-5714. https://doi.org/10.1093/emboj/20.20.5703

Journal Article Type Article
Publication Date Oct 1, 2001
Deposit Date Jan 9, 2009
Journal EMBO Journal
Print ISSN 0261-4189
Electronic ISSN 1460-2075
Publisher EMBO Press
Peer Reviewed Not Peer Reviewed
Volume 20
Issue 20
Pages 5703-5714
DOI https://doi.org/10.1093/emboj/20.20.5703
Keywords NPC, Nuclear basket, Nuclear import, Nuclear pore complex, Nup153.