A Combined Spectrophotometer and Fluorometer to Demonstrate the Principles of Absorption Spectroscopy

Lietard, Aude; Screen, Martin A.; Flindt, Diana L.; Jordan, Caleb J.C.; Robson, Jacquie M.; Verlet, Jan R.R.

doi:10.1021/acs.jchemed.1c00742

A Combined Spectrophotometer and Fluorometer to Demonstrate the Principles of Absorption Spectroscopy

Lietard, Aude; Screen, Martin A.; Flindt, Diana L.; Jordan, Caleb J.C.; Robson, Jacquie M.; Verlet, Jan R.R.

Authors

Aude Lietard

Martin A. Screen

Diana L. Flindt

Caleb J.C. Jordan

Professor Jacquie Robson j.m.robson@durham.ac.uk
Professor

Professor Jan Verlet j.r.r.verlet@durham.ac.uk
Professor

Abstract

A dual-function student-crafted instrument is described as part of a laboratory activity aimed to teach both the principles and practical aspects of absorption spectroscopy to secondary and introductory undergraduate students. Using minimal changes in an arrangement that is based on interlocking bricks and low-cost components, both a fluorometer and photometer have been constructed. The former demonstrates the principles of the Beer–Lambert law visually and quantitatively by acquiring the spatial light attenuation through a fluorescent sample. The latter then demonstrates its practical application in a visible-light spectrometer by measuring the absorption spectrum of an aqueous permanganate solution.

Citation

Lietard, A., Screen, M. A., Flindt, D. L., Jordan, C. J., Robson, J. M., & Verlet, J. R. (2021). A Combined Spectrophotometer and Fluorometer to Demonstrate the Principles of Absorption Spectroscopy. Journal of Chemical Education, 98(12), 3871-3877. https://doi.org/10.1021/acs.jchemed.1c00742

Journal Article Type	Article
Acceptance Date	Nov 1, 2021
Online Publication Date	Nov 12, 2021
Publication Date	Dec 14, 2021
Deposit Date	Jan 11, 2022
Publicly Available Date	Nov 12, 2022
Journal	Journal of Chemical Education
Print ISSN	0021-9584
Electronic ISSN	1938-1328
Publisher	American Chemical Society
Peer Reviewed	Peer Reviewed
Volume	98
Issue	12
Pages	3871-3877
DOI	https://doi.org/10.1021/acs.jchemed.1c00742
Public URL	https://durham-repository.worktribe.com/output/1217516

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Copyright Statement
This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Chemical Education, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.jchemed.1c00742