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A Combined Spectrophotometer and Fluorometer to Demonstrate the Principles of Absorption Spectroscopy

Lietard, Aude and Screen, Martin A. and Flindt, Diana L. and Jordan, Caleb J. C. and Robson, Jacquie M. and Verlet, Jan R. R. (2021) 'A Combined Spectrophotometer and Fluorometer to Demonstrate the Principles of Absorption Spectroscopy.', Journal of Chemical Education, 98 (12). pp. 3871-3877.


A dual-function student-crafted instrument is described as part of a laboratory activity aimed to teach both the principles and practical aspects of absorption spectroscopy to secondary and introductory undergraduate students. Using minimal changes in an arrangement that is based on interlocking bricks and low-cost components, both a fluorometer and photometer have been constructed. The former demonstrates the principles of the Beer–Lambert law visually and quantitatively by acquiring the spatial light attenuation through a fluorescent sample. The latter then demonstrates its practical application in a visible-light spectrometer by measuring the absorption spectrum of an aqueous permanganate solution.

Item Type:Article
Full text:(AM) Accepted Manuscript
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Publisher statement:This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Chemical Education, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see
Date accepted:No date available
Date deposited:11 January 2022
Date of first online publication:12 November 2021
Date first made open access:12 November 2022

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