Skip to main content

Research Repository

Advanced Search

Enhancement of sensitivity for dichlorvos detection by a low-weight gelator based on bolaamphiphile amino acid derivatives decorated with a hybrid graphene quantum dot/enzyme/hydrogel

Sahub, Chonticha; Andrews, Jessica L.; Smith, James P.; Mohamad Arif, Maya A.; Tomapatanaget, Boosayarat; Steed, Jonathan W.

Enhancement of sensitivity for dichlorvos detection by a low-weight gelator based on bolaamphiphile amino acid derivatives decorated with a hybrid graphene quantum dot/enzyme/hydrogel Thumbnail


Authors

Chonticha Sahub

Jessica L. Andrews

James P. Smith

Maya A. Mohamad Arif

Boosayarat Tomapatanaget



Abstract

Developing the sensitivity of an optical biosensor motivates the exploration of an amplification strategy for the incorporation of a biosensor system in a supramolecular hydrogel. We developed novel fluorescent hybrid materials comprising graphene quantum dots and enzymes supported by L-phenylalanine derived bis(urea) supramolecular hydrogels (GQDs/Enz/Gels) for detection of organophosphates. The addition of acetylcholinesterase (AChE) and choline oxidase (ChOx) during the formation of self-assembled GQDs/Gel materials resulted in enzyme-functionalized gel networks. Significant turn-off fluorescence of the encapsulated GQDs in the hydrogels was due to hydrogen peroxide generated from the active enzymatic reaction. The addition of insecticide dichlorvos to the GQDs/Enz/Gels resulted in the recovery of fluorescence in proportion to the concentration of dichlorvos, with a detection limit of 2.61 × 10−8 M which outperforms those in solution by 100-fold improvement and a wider linear range of 1.25 × 10−8–1.25 × 10−4 M compared to those of the biosensor in solution. These hybrid hydrogels show promising sensitivity to the detection of oxy-form organophosphate pesticides and expectedly offer scope for the development of rapid and environmentally friendly techniques.

Citation

Sahub, C., Andrews, J. L., Smith, J. P., Mohamad Arif, M. A., Tomapatanaget, B., & Steed, J. W. (2021). Enhancement of sensitivity for dichlorvos detection by a low-weight gelator based on bolaamphiphile amino acid derivatives decorated with a hybrid graphene quantum dot/enzyme/hydrogel. Materials Chemistry Frontiers, 5(18), 6850-6859. https://doi.org/10.1039/d1qm00296a

Journal Article Type Article
Acceptance Date Jul 7, 2021
Online Publication Date Jul 9, 2021
Publication Date Sep 21, 2021
Deposit Date Jan 11, 2022
Publicly Available Date Mar 29, 2024
Journal Materials Chemistry Frontiers
Print ISSN 2052-1537
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 5
Issue 18
Pages 6850-6859
DOI https://doi.org/10.1039/d1qm00296a

Files





You might also like



Downloadable Citations