Sangita Das
Human peripheral blood mononuclear cells targeted multidimensional switch for selective detection of HSO3− anion
Das, Sangita; Das, Partha Pratim; Walton, James W.; Ghoshal, Kakali; Patra, Lakshman; Bhattacharyya, Maitree; Mondal, Tapan Kumar; Thomas, Sabu
Authors
Partha Pratim Das
Dr James Walton james.walton@durham.ac.uk
Associate Professor
Kakali Ghoshal
Lakshman Patra
Maitree Bhattacharyya
Tapan Kumar Mondal
Sabu Thomas
Abstract
A new ratiometric π-conjugated luminophore with donor-acceptor (D- π- A) network CM {(E)-2-(4-(2-(9-butyl-9H-carbazol-3-yl)vinyl)benzylidine)malononitrile} has been synthesized by malononitrile conjugated carbazole dye with an intervening p-styryl spacer. Here, p-styryl conjugated malononitrile is used as a recognition site for the detection of HSO3− with a fast response time (within 50 s). In a mixed aqueous solution, CM reacts with HSO3− to give a new product 1-(9-butyl-9H-carbazol-3-yl)-2-(4-(2, 2-dicyanovinyl)phenyl)ethane-1-sulfonic acid. The probe exhibits positive solvatofluorochromism with solid state red fluorescence. The restriction of intermolecular rotation of p-styryl conjugated malononitrile unit enhances the typical solid state fluorescence properties. The probe (CM and its corresponding aldehyde CA) also demonstrates a strong solvent dependence yielding blue to green to pink and even red fluorescence in commonly used organic solvents like n-hexane, toluene, diethyl ether (DEE), THF, DCM, Dioxane, CH3CN and MeOH. The chemodosimetric approach of HSO3− selectively takes place at the olefinic carbon exhibiting a prominent chromogenic as well as ratiometric fluorescence change with a 147 nm blue-shift in the fluorescence spectrum. CM can detect HSO3− as low as 1.21 × 10−8 M. Moreover, the CM can be successfully applied to detect intrinsically generated intracellular HSO3− in human peripheral blood mononuclear cells (PBMCs). CM has shown sharp intensities (2628 ± 511.8) when the cells are HSO3− untreated. At green channel (at 486 nm) almost negligible fluorescence intensities are found (423 ± 127.5) for HSO3− untreated samples. However, the green fluorescence (2863 ± 427.5) increases significantly (p < 0.05), and simultaneously the red fluorescence gets significantly (p < 0.05) diminished (515 ± 113.2) after addition of HSO3−. The CM has been effectively utilized for evaluating the bisulfite ions in food samples as well. The concentrations of HSO3− in diluted sugar samples have been determined with the recovery of 97.6–9.12%.
Citation
Das, S., Das, P. P., Walton, J. W., Ghoshal, K., Patra, L., Bhattacharyya, M., …Thomas, S. (2022). Human peripheral blood mononuclear cells targeted multidimensional switch for selective detection of HSO3− anion. Dyes and Pigments, 198, Article 109966. https://doi.org/10.1016/j.dyepig.2021.109966
Journal Article Type | Article |
---|---|
Acceptance Date | Nov 21, 2021 |
Online Publication Date | Nov 25, 2021 |
Publication Date | 2022-02 |
Deposit Date | Jan 13, 2022 |
Publicly Available Date | Nov 25, 2022 |
Journal | Dyes and Pigments |
Print ISSN | 0143-7208 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 198 |
Article Number | 109966 |
DOI | https://doi.org/10.1016/j.dyepig.2021.109966 |
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Copyright Statement
© 2021 This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
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