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Realtime wavefront sensing in a SPIM microscope, and active aberration tracking

Taylor, Jonathan M. and Saunter, Christopher D. and Bourgenot, Cyril and Girkin, John M. and Love, Gordon D. (2015) 'Realtime wavefront sensing in a SPIM microscope, and active aberration tracking.', Adaptive Optics and Wavefront Control for Biological Systems San Francisco, CA, 07-12 Feb 2015.


Adaptive optics (AO) can potentially allow high resolution imaging deep inside living tissue, mitigating against the loss of resolution due to aberrations caused by overlying tissue. Closed-loop AO correction is particularly attractive for moving tissue and spatially varying aberrations, but this requires direct wavefront sensing, which in turn requires suitable "guide stars" for use as wavefront references. We present a novel method for generating an orthogonally illuminated guide star suitable for direct wavefront sensing in a wide range of fluorescent biological structures, along with results demonstrating its use for measuring time-varying aberrations, in vivo.

Item Type:Conference item (Paper)
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Publisher statement:Copyright 2015 Society of Photo Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
Date accepted:No date available
Date deposited:22 April 2022
Date of first online publication:31 March 2015
Date first made open access:22 April 2022

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