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Adaptive optics for wide-field microscopy

Bourgenot, C.; Saunter, C.D.; Girkin, J.M.; Love, G.D.

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Authors

C.D. Saunter

G.D. Love



Abstract

We report on recent developments in the use of adaptive optics (AO) in wide-field microscopy to remove both system and sample induced aberrations. We describe progress on using both a full AO system and image optimization techniques (wavefront sensorless AO). In the latter system the determination of the best mirror shape is found via two routes. In the first an optimization algorithm using a Simplex search pattern is used with an initial random set of mirror shapes. We then explore the use of specific Zernike terms as our starting basis set. In both cases the final optimization performance is not affected by the choice of optimization metric. We then describe an open loop AO system in which the equivalent of a laser guide star is used as the light source for the wavefront sensor.

Citation

Bourgenot, C., Saunter, C., Girkin, J., & Love, G. (2011). Adaptive optics for wide-field microscopy. . https://doi.org/10.1117/12.873857

Conference Name Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII
Conference Location San Francisco, CA
Start Date Jan 22, 2011
End Date Jan 27, 2011
Online Publication Date Feb 23, 2011
Publication Date 2011
Deposit Date Feb 13, 2018
Publicly Available Date Apr 22, 2022
Volume 7904
Series ISSN 0277-786X
DOI https://doi.org/10.1117/12.873857

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Copyright Statement
Copyright 2011 Society of Photo Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.





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