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Adaptive optics for wide-field microscopy

Bourgenot, C. and Saunter, C. D. and Girkin, J. M. and Love, G. D. (2011) 'Adaptive optics for wide-field microscopy.', Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII San Francisco, CA, 22-27 Jan 2011.

Abstract

We report on recent developments in the use of adaptive optics (AO) in wide-field microscopy to remove both system and sample induced aberrations. We describe progress on using both a full AO system and image optimization techniques (wavefront sensorless AO). In the latter system the determination of the best mirror shape is found via two routes. In the first an optimization algorithm using a Simplex search pattern is used with an initial random set of mirror shapes. We then explore the use of specific Zernike terms as our starting basis set. In both cases the final optimization performance is not affected by the choice of optimization metric. We then describe an open loop AO system in which the equivalent of a laser guide star is used as the light source for the wavefront sensor.

Item Type:Conference item (Paper)
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Status:Peer-reviewed
Publisher Web site:https://doi.org/10.1117/12.873857
Publisher statement:Copyright 2011 Society of Photo Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
Date accepted:No date available
Date deposited:22 April 2022
Date of first online publication:23 February 2011
Date first made open access:22 April 2022

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