Closed loop adaptive optics with a laser guide star for biological light microscopy

Saunter, Christopher D.; Bourgenot, Cyril; Girkin, John M.; Love, Gordon D.

doi:10.1117/12.909927

Closed loop adaptive optics with a laser guide star for biological light microscopy

Saunter, Christopher D.; Bourgenot, Cyril; Girkin, John M.; Love, Gordon D.

Authors

Christopher D. Saunter

Cyril Bourgenot cyril.bourgenot@durham.ac.uk
Associate Professor

Professor John Girkin j.m.girkin@durham.ac.uk
Professor

Gordon D. Love

Contributors

Scot S Oliver
Editor

Thomas G Bifano
Editor

Joel Kubby
Editor

Abstract

We report on the development of a widefield microscope that achieves adaptive optics correction through the use of a wavefront sensor observing an artificial laser guide star induced within the sample. By generating this guide star at arbitrary positions and depths within the sample we allow the delivery of high-resolution images. This approach delivers much faster AO correction than image optimization techniques, and allows the use of AO with fluorescent imaging modalities without generating excessive photo-toxic damage in the sample, or inducing significant photo-bleaching in the flurophore molecules.

Citation

Saunter, C. D., Bourgenot, C., Girkin, J. M., & Love, G. D. (2012). Closed loop adaptive optics with a laser guide star for biological light microscopy. In S. S. Oliver, T. G. Bifano, & J. Kubby (Eds.), . https://doi.org/10.1117/12.909927

Conference Name	MEMS Adaptive Optics VI
Conference Location	San Francisco, CA
Start Date	Jan 21, 2012
End Date	Jan 26, 2012
Online Publication Date	Feb 15, 2012
Publication Date	2012
Deposit Date	Feb 13, 2018
Publicly Available Date	Apr 22, 2022
Volume	8253
Series ISSN	0277-786X
DOI	https://doi.org/10.1117/12.909927
Public URL	https://durham-repository.worktribe.com/output/1145835

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Copyright 2012 Society of Photo Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.