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Closed loop adaptive optics with a laser guide star for biological light microscopy

Saunter, Christopher D. and Bourgenot, Cyril and Girkin, John M. and Love, Gordon D. (2012) 'Closed loop adaptive optics with a laser guide star for biological light microscopy.', MEMS Adaptive Optics VI San Francisco, CA, 21-26 Jan 2012.


We report on the development of a widefield microscope that achieves adaptive optics correction through the use of a wavefront sensor observing an artificial laser guide star induced within the sample. By generating this guide star at arbitrary positions and depths within the sample we allow the delivery of high-resolution images. This approach delivers much faster AO correction than image optimization techniques, and allows the use of AO with fluorescent imaging modalities without generating excessive photo-toxic damage in the sample, or inducing significant photo-bleaching in the flurophore molecules.

Item Type:Conference item (Paper)
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Publisher statement:Copyright 2012 Society of Photo Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
Date accepted:No date available
Date deposited:22 April 2022
Date of first online publication:15 February 2012
Date first made open access:22 April 2022

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