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GhTCE1-GhTCEE1 dimers regulate transcriptional reprogramming during wound-induced callus formation in cotton

Deng, J.; Sun, W.; Zhang, B.; Sun, S.; Xia, L.; Miao, Y.; He, L.; Lindsey, K.; Yang, X.; Zhang, X.

GhTCE1-GhTCEE1 dimers regulate transcriptional reprogramming during wound-induced callus formation in cotton Thumbnail


Authors

J. Deng

W. Sun

B. Zhang

S. Sun

L. Xia

Y. Miao

L. He

X. Yang

X. Zhang



Abstract

Wounded plant cells can form callus to seal the wound site. Alternatively, wounding can cause adventitious organogenesis or somatic embryogenesis. These distinct developmental pathways require specific cell fate decisions. Here, we identify GhTCE1, a basic helix-loop-helix (bHLH) family transcription factor, and its interacting partners as a central regulatory module of early cell fate transition during in vitro dedifferentiation of cotton (Gossypium hirsutum). RNAi- or CRISPR/Cas9-mediated loss of GhTCE1 function resulted in excessive accumulation of reactive oxygen species (ROS), arrested callus cell elongation, and increased adventitious organogenesis. In contrast, GhTCE1-overexpressing tissues underwent callus cell growth, but organogenesis was repressed. Transcriptome analysis revealed that several pathways depend on proper regulation of GhTCE1 expression, including lipid transfer pathway components, ROS homeostasis and cell expansion. GhTCE1 bound to the promoters the target genes GhLTP2 and GhLTP3, activating their expression synergistically, and the heterodimer TCE1-TCEE1 enhances this activity. GhLTP2- and GhLTP3-deficient tissues accumulated ROS and had arrested callus cell elongation, which was restored by ROS scavengers. These results reveal a unique regulatory network involving ROS and lipid transfer proteins, which act as potential ROS scavengers. This network acts as a switch between unorganized callus growth and organized development during in vitro dedifferentiation of cotton cells.

Citation

Deng, J., Sun, W., Zhang, B., Sun, S., Xia, L., Miao, Y., …Zhang, X. (2022). GhTCE1-GhTCEE1 dimers regulate transcriptional reprogramming during wound-induced callus formation in cotton. The Plant Cell, 34(11), 4554-4568. https://doi.org/10.1093/plcell/koac252

Journal Article Type Article
Acceptance Date Aug 2, 2022
Online Publication Date Aug 16, 2022
Publication Date 2022-11
Deposit Date Aug 18, 2022
Publicly Available Date Aug 18, 2022
Journal Plant Cell
Print ISSN 1040-4651
Electronic ISSN 1532-298X
Publisher Oxford University Press
Peer Reviewed Peer Reviewed
Volume 34
Issue 11
Pages 4554-4568
DOI https://doi.org/10.1093/plcell/koac252

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Publisher Licence URL
http://creativecommons.org/licenses/by-nc-nd/4.0/

Copyright Statement
© The Author(s) 2022. Published by Oxford University Press on behalf of American Society of Plant Biologists.
This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com






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